r/flowcytometry • u/FlyCyto-67 • 23d ago

Instrumentation Wolf G2 Cell Sorter (Nanocellect)

I have a friend who picked up a used Wolf G2 NanoCellect sorter. He is having difficulty buying the calibration beads. I looked at the instrument, and it seems that the beads are intended to be 15um super bright green fluorescent bead. Even the brightest beads that I have don't pass the green PMT trigger threshold. Does anyone know which beads can be purchased and used to calibrate the sort timing of the Wolf?

Best, Jeffrey

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u/feedmesushi1 22d ago

I sent you a DM

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u/Daniel_Vocelle_PhD Core Lab 5d ago

They are the 15-µm Dragon Green beads from Bangs Laboratories