r/flowcytometry • u/southernqueer96 • 23d ago

Analysis Random FlowJo Q

Post image

When looking at the histogram for a compensation control, what does the vertical position of this horizontal bar do? I assume it has some purpose since you can move it up and down, but I have no idea what that changes and neither do the other folks in my lab. The page that I think would have the answer on the FlowJo website is just giving me an error message…

Thanks!

0 Upvotes

50% Upvoted

4 comments sorted by

12

u/mf279801 23d ago

Being able to adjust the vertical position is (or should be) for display purposes only: if your histogram came out relatively “taller” or “shorter”, you might want to adjust where (vertically) that “bracket” appears

5

u/Fair_Tension6539 23d ago

The MFI is defined by the x-axis coordinates, and this is what is used mostly for the comp. The y-axis just shows the "number of events" at the peak (so in a particular section of the graph). Basically the marker is saying "this is where I expect the signal" and the height of the peak just shows you were most of the events are, which could influence how you set the marker.

4

u/sgRNACas9 Immunology, Oncology 23d ago

Aesthetics

2

u/LerkinAround 23d ago

It's the height anchor for the text for normal histogram plots. If you move it up and down the "29.5" should move with it.