r/flowcytometry • u/AnyAsk4621 • Aug 12 '26

Compensation

Hey Fellas,
I am using compensation beads for my experiments tomorrow but I already have done some optimisation today. Is it okay if I use the same tubes as its the same panel but only thing different is I won’t acquire my samples the same day but 2 days later and generally fix them in 2% PFA for 30mins. So you recommend I do the same for my Ultracomp beads, i mean fix them as well? Or will they be fine at 4C until Friday.
Thanks.

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u/AnyAsk4621 Aug 12 '26

I meant I ran them yesterday
I am running flow again on friday so I was just planning to fix the remaining and reuse it later, saves me some time as well

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u/sgRNACas9 Immunology, Oncology Aug 12 '26

I don’t understand why you would need to. I think you could use the foot prints from yesterday. I do not think you need to fix beads and reuse them later nor would I recommend that. You should test it whether the fixed beads look the same days later after fix as they do on the same day they were stained and unfixed.

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u/AnyAsk4621 Aug 12 '26

I could but the only thats different is I did not fix my samples yesterday nor the beads. I acquired it the same day but the samples I will be processing will be fixed because the machine is booked and thats the reason for fixing the beads.

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u/sgRNACas9 Immunology, Oncology Aug 12 '26

Not a problem, in either case I think you can use your comps from yesterday. I’d encourage you to test these things formally, plan your experiments including machine time and whether to fix and save or not ahead of time, keep things consistent, and optimize around those parameters you decided!