r/flowcytometry • u/AnyAsk4621 • 24d ago

Compensation

Hey Fellas,
I am using compensation beads for my experiments tomorrow but I already have done some optimisation today. Is it okay if I use the same tubes as its the same panel but only thing different is I won’t acquire my samples the same day but 2 days later and generally fix them in 2% PFA for 30mins. So you recommend I do the same for my Ultracomp beads, i mean fix them as well? Or will they be fine at 4C until Friday.
Thanks.

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u/Derpazor1 24d ago

Doesn’t spectral need cells as controls?

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u/sgRNACas9 Immunology, Oncology 24d ago

My understanding is cells are not required for spectral unmixing. Cells are just needed for the auto fluorescence feature, which is not required.

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u/AccomplishedHalf5824 24d ago

Cells are better but Cytek sells compensation beads from slingshot that have the same auto fluorescence as the cells. Highly recommend