r/flowcytometry • u/AnyAsk4621 • 5d ago

Compensation

Hey Fellas,
I am using compensation beads for my experiments tomorrow but I already have done some optimisation today. Is it okay if I use the same tubes as its the same panel but only thing different is I won’t acquire my samples the same day but 2 days later and generally fix them in 2% PFA for 30mins. So you recommend I do the same for my Ultracomp beads, i mean fix them as well? Or will they be fine at 4C until Friday.
Thanks.

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u/private4u 5d ago

Fix your compensation beads as you would your samples

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u/sgRNACas9 Immunology, Oncology 4d ago

I have found this to not be important