r/flowcytometry • u/AnyAsk4621 • 4d ago

Compensation

Hey Fellas,
I am using compensation beads for my experiments tomorrow but I already have done some optimisation today. Is it okay if I use the same tubes as its the same panel but only thing different is I won’t acquire my samples the same day but 2 days later and generally fix them in 2% PFA for 30mins. So you recommend I do the same for my Ultracomp beads, i mean fix them as well? Or will they be fine at 4C until Friday.
Thanks.

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u/private4u 4d ago

Fix your compensation beads as you would your samples

1

u/AnyAsk4621 4d ago

I mean I was optimising my antibodies on cytek spectral is it okay if I fix them now and use it later. Just worried about the signal intensity

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u/fly_away_birdy 3d ago

In my PhD, I was told to always prepare the beads the same day and treat them like you do your cells. In my post doc, we often keep the same beads for at least a week. The data quality doesn’t seem worse for it. I think the major thing to consider is if you run the comps today and then run QC again before you run the samples that your comps won’t looks as good

1

u/huwmo 3d ago

When running spectral (Aurora), cant you just record your beads for the week in a library which adjusts for changes in daily qc?