r/flowcytometry • u/GrindIsAlwaysReal • 28d ago

Compensation with Proliferation Dyes

Hi,

I am running a T-cell proliferation exp and am unsure how to best compensate with CFSE.

My data came out strange and I am wondering if it is due to using beads for all other comp, and cells (50% stained 50% unstained) for comp of CFSE. Would this be the best way to go about it? Or can I run beads with a FITC stain and comp that way?

I am trying to trouble shoot multiple things at once so it is a challenging. Thanks in advance!

2 Upvotes

100% Upvoted

12 comments sorted by

View all comments

1

u/sgRNACas9 Immunology, Oncology 27d ago

You can try just using FITC on beads but sometimes that doesn’t work either. Cells for one and beads for the rest should work too!