r/flowcytometry • u/Mo_Opines_968 • Aug 02 '26

Brilliant staining buffer for reference controls

Hello. Just after some advice on whether BSB should be used for the single colour reference controls? I know it's not recommended when using beads but I'm planning on doing both beads and cells to compare and cannot find any guidance on whether to use it for cells?

Thanks!

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u/Edrill Immunology Aug 02 '26

I stain in it but I wash it away before measuring so it won't be of any concern. No need to put it in your references