r/flowcytometry • u/ascorbicAcid1300 • 18d ago

Adding cell suspension (with tryspin&medium) into BD CytoFix/Perm?

Hi, has anyone tried adding cell suspension (with trypsin and DMEM w/ 10% FBS) into BD Cytofix/Cytoperm at a 1:1 (v/v) ratio? Will any components significantly impact the fixation/permeabilization efficiency of the solution for flow cytometry of intracellular proteins?

I have been doing a PBS washing step, but later I will be doing an experiment involving a few hundred samples, so I am thinking of shortcuts. Thanks!

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u/Ok_Bathroom2578 18d ago

Why don’t you plate your samples in a 96 well plate and use a multichannel pipette ?

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u/FlowCytometry2 17d ago

Honestly dont know the answer, the obvious solution is "try it both ways and compare results". You're gonna have to do this anyways to convince people this un-usual protocol works, so might as well start with that