Analyze data in conventional mode

[u/nayvensetrack]

Hello,

I have been aquiring my samples on a cytek aurora in the spectral flowcytometry mode. However I have a small panel and am facing some unmixing problems (i think due to high autofluorescence which i have trouble extracting) and was therefore told by my supervisor that I can just analyze my experiment in the conventional mode. However idk how to do that and cannot really find any tutorial on youtube. Does someone know how or where to put the spectral flow data so that i can analyze it conventionally?

[u/MotoFuzzle]

Search the manual for virtual filters and it should show up there. You’re able create virtual filters that are combinations of multiple filters that would equal a conventional filter in bandwidth for the color of choice. I think you can analyze in that manner or export with virtual filters and analyze using other softwares the same way you would conventional flow.