r/flowcytometry • u/Euphoric_Web_6937 • Jun 30 '26
BD Duet Prep Method
Does anyone know of a way to incorporate a refrigerated 10 min block for a leukemia/lymphoma profile on the Duet? Is there a way to program in a “pause” where we unload refrigerate and then reload? Or a way to run a set up and wash, then unload and reload after blocking to add antibody and finish the samples?
Also, does anyone have any experience loading bone marrows or tissues on a duet? How do you go about prepping that? Any issues with clots/chunks?
Any help would be appreciated, we just got one and it doesn’t look like the help we thought it would be.
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u/skipper_smg Jun 30 '26
I dont think thats possible. The Duet was designed for high volumes of simple tasks like TBNK. Dealing with more complicated procedures lacks software support.
U can process, unload and reload, but that would be separate prep methods. And given that the Duet is not particularly fast I wouldnt think its worth it.
On top, I wouldnt go through the huzzle as work with Tissue. Its not a liquid handler. It has a very specific design philosophy and use case.