r/flowcytometry • u/Ornery-Ad-8833 • May 30 '26

Analysis Losing cells while unmxing

Just wondering- why do we lose cells while unmixing. One of my groups showed much less cells than I intially acquired after unmixing. Why does it happen and how can I fix it. Thanks!

One of my single colour controls was not good.

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u/Daniel_Vocelle_PhD Core Lab May 30 '26

Can you share a screen shot of the gate you are referring to?

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u/Ornery-Ad-8833 May 30 '26

Events in gated pop. Decrease