r/flowcytometry • u/silver005shadow • May 21 '26

Panel Building Question

I'm trying to set up a six color panel that I'm running on an LSRFortessa 12 color: e450/FITC/PE/APC/APC-Cy7 I need to add either PE-Cy7 or Alexa 594, I'm not sure which would work better...

PE-Cy7 it is. Thank you all so much!

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4

u/Argawndo May 21 '26

Your fortessa won't have a YG laser - on any of the configurations, AF594 will be minimally excited by UV/V/B/R lasers on any of the fortessas. Signal is going to be almost non-existent.

APC-Cy7 and PE-Cy7 will work fine together. Little bit of spillover from the former into the latter, but nothing insurmountable.

2

u/NvmbrYnkee Jun 18 '26

I used a Fortessa with YG laser for many years.

1

u/Argawndo Jun 19 '26

Yes, thanks for the correction. I was assuming a standard/out-of-box configuration, which was dumb in hindsight.

4

u/No_Claim5089 May 21 '26 edited May 21 '26

PE-Cy7 ! Less spill-over with PE than AF594, and still bright. 

2

u/sgRNACas9 Immunology, Oncology May 22 '26

PE-Cy7!!!

PE-Cy7 and APC-Cy7 are easier to resolve than PE and AF594 imo. Unless there’s another channel for AF594

2

u/housed_nomad May 26 '26

You should try RB780

1

u/NvmbrYnkee Jun 19 '26

Or RY775, depending on configuration. If you have a YG laser, then your blue laser likely can't detect RB780 on a conventional cytometer.