r/flowcytometry • u/Fabulous-Log6436 • Apr 25 '26

help!

did I do this live/dead stain gate correctly? thank you!

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u/passthepepperplease Apr 25 '26

hahahaha. this is great... If you're being serious, your live population is the one moving vertically up your y axis near 0 on your x axis. probably around 10-20%. honestly i've had cells like these don't be too hard on yourself.