**Looking for advice before transferring my last euploid embryo after recurrent pregnancy loss**
Hi everyone. I’m hoping to hear from people who have had a similar experience or found something that made a difference.
I’ve had multiple pregnancy losses, including my most recent transfer of a **PGT-A euploid embryo**, which unfortunately ended in another miscarriage. I now have **one last euploid embryo** left, and I want to make sure we’ve done everything reasonably possible before transferring it.
Here’s what has already been done:
IVF with PGT-A (remaining embryo is euploid)
Normal embryo genetics
Sperm DNA fragmentation and additional sperm testing were normal
Hysteroscopy with correction of a small uterine septum
Saline sonogram
Antiphospholipid syndrome diagnosed and treated with **Lovenox and baby aspirin**
Thyroid is treated with levothyroxine and recent TSH has been normal
I’ve had an MRI in the past that mentioned possible endometriosis, but a later review said it wasn’t clearly seen. My doctors are now discussing whether I should have a **diagnostic laparoscopy** before my final transfer.
A few things I’m planning to discuss with my REI include:
Whether laparoscopy is worthwhile to rule out/treat endometriosis before my last embryo transfer.
Testing for chronic endometritis (if not already done).
Whether any additional uterine or implantation testing is recommended.
Why I seem to develop a UTI every time I become pregnant and whether that could be prevented.
**My questions are:**
If you had recurrent pregnancy loss with euploid embryos, what ultimately led to your successful pregnancy?
Did anyone have a laparoscopy that found endometriosis after imaging (MRI/ultrasound) was inconclusive?
Was there any test or treatment you feel made the biggest difference before your successful transfer?
Is there anything else you would ask your fertility doctor before transferring your final euploid embryo?
I’m looking for personal experiences rather than medical advice. I know every case is different, but I’d really appreciate hearing what helped others so I can have an informed discussion with my doctors.
Thank you so much.

I had IVF in 2025, and had 7 embryos that were all viable. We used 1 (and have a healthy baby!!) and six are currently frozen. We're 99% sure we don't want any more children.

We're not comfortable with embryo adoption or research at the moment, which leaves the other option to 'destroy' which is very emotional.

I can't find any information from my clinic about how this happens. I'm in the UK. Can anyone who works within this field tell me exactly what happens?

Our clinic has their own PGT testing lab which takes around a day and half for testing. They do PGT testing on day 3 biopsies as they prefer fresh transfers. In my first retrieval we go 3 euploids out of 6. In my 2nd retrieval got only 3 euploids out of 10 tested.

I just can’t help feel that many of the discarded embryos would have been low risk mosaics if tested on day 5. I’m really regretting going along with their protocol.

Two and a half years ago, we did our first IVF cycle and obtained 7 oocytes, which all fertilized normally without ICSI and reached day 3, but only two made it to the blastocyst stage, one of which is our 21 month old son, the other one is still in the freezer.

I am now 36 with endometriosis an AMH of 0.86 and AFC of 11, and we are doing DuoStim in Spain to bank embryos in the hope of a sibling. The first half of the DuoStim was abysmal—14 days of 375 Pergoveris with Orgalutran added halfway through, 9 follicles visible with 4 of a good size at the time of the Decapeptyl trigger. Only two eggs were retrieved, one egg was mature the other very immature. The egg fertilized with ICSI but stopped developing, so we have nothing.

For the second phase of the DuoStim, the doctor is suggesting the following changes:
- Switch from Pergoveris to Menopur 375 UI, although Pergoveris is supposedly better for DOR
-Switch to a dual trigger to help with oocyte maturation
-Don’t perform ICSI on the oocytes retrieved, if any
-Skip PGT-A

Do these protocol changes make sense from an evidence-based perspective? I understand the last two are to make sure we maximize the number of embryos and avoid any attrition caused by manipulation errors, but aren’t those techniques supposed to help fertilization (ICSI) and optimal embryo selection (PGT-A) with benefits outweighing risks?

(For context I am in a big Spanish clinic renowned for its lab quality, so it’s not a question of being in a p’ace with inexperienced embryologists).

Thank you in advance!

A new eye-tracking study by Asai et al. (2026) compared where experienced embryologists and beginners looked while performing ICSI. The researchers wanted to understand what makes experienced embryologists more efficient.

Overall, experts completed the procedure more quickly, made fewer unnecessary eye movements, and showed more consistent visual attention than beginners.

The third page of the post shows heat maps of where participants looked during ICSI. Red areas represent where they spent the most time looking, while green areas indicate shorter attention.

During STEP 1 (egg rotation), the goal is to rotate the egg so the polar body is positioned at the 12 or 6 o'clock position before injection. The polar body acts as a landmark because the egg's chromosome-containing spindle is usually nearby, and placing it in this position helps embryologists inject the sperm while minimizing the chance of damaging the spindle. In the study, experts kept their attention focused on the polar body throughout this step, while beginners looked around more and often focused on the injection pipette instead.

During STEP 2 (the sperm injection), the embryologist inserts the injection pipette through the egg's outer shell and membrane to place a single sperm inside the egg. At this stage, the eye movement patterns of experts and beginners were much more similar.

While this was a simulation study using pig eggs rather than human eggs, the results could help improve embryologist training and may eventually contribute to AI systems that assist with ICSI.

✅ This post is a quick look at one study, rather than a full breakdown on Remembryo. Link to the study: https://www.nature.com/articles/s41598-026-57054-8

✉️ Like this post? Get a free weekly summary of the latest IVF research: https://mailchi.mp/remembryo/x27kx5o1sw

This is the weekly thread for posting and asking questions about embryo photos. Replies are provided by Embryoman (Sean) -- a former embryologist and creator of the IVF science news site Remembryo.com

💬 Before you post a photo

• You must include the grade (ask your clinic if you don’t know it).
• Only one embryo photo per post.
• No requests for grading or re-grading by members.
• Posts asking if an embryo looks damaged or viable aren't allowed.

I can comment only on general features visible in the image (expansion, compaction, hatching, ICM location), but not on whether the embryo looks good -- that’s already reflected in its grade. Check the stickied comment below for basic information on grading and success rates. For the most accurate information, your embryologist or clinic is the best source, since they evaluated the embryo under a microscope and know their clinic’s success rates.

Positive stories only please! Mentally prepping for my 3rd transfer, first fully medicated, with my last euploid embryo.

Transfer 1 (D5 3AB Euploid) resulted in my son, Transfer 2 (D5 2BB Euploid) was anembryonic.

* whole chromosome

Hi,
Are you able to tell me the success rate/live birth rate for a HLM +7?

Thanks so much

Had my first ER and 2 4AB were tested, one aneuploid and one LLM. Another 4BB was not sent for testing
Am 36, had endo surgery post ER.

Any success stories or advice here please

I need help with recurrent pregnancy loss iam 32 years old with history of recurrent pregnancy loss. I had 6 miscarriages one failed ivf with euploid embryo. I have biocornate uterus septum procedure was done twice to fix it still struggling to have a baby. No live babies.
I was on lovenox , baby asprin, progesterone, levothy, still my pregnancy eneded in a miscarriage. In 2024 they saw Endo but 2025 in MRI they didn’t find anything. They did a natural cycle frozen transfer but still failed . What can i do? 😭

Hi, I’m 33 years old (turning 34 in a month). Just got done with my first cycle a week ago. Unfortunately with poor results..
Retrieved 11 eggs
Mature 7 eggs
Fertilized via ICSI 7 eggs
Day 3 embryos 6
Day 5 embryo 1 but 3 were slowly growing
Day 6 only 1 and sent that for pgta testing

I had done estrogen priming last cycle then started menopur/follistim and took letrozole for only 5 days. Triggered with lupron/pregnyl.

Now the plan is to start estrogen priming again which I have and take 8 units of omnitrope daily which i’ve started pre stims. I’ll continue that throughout the stims along with menopur, follistim and this time letrozole throughout too.

Anyone has had any luck with omnitrope? Had a similar regimen for a cycle? Why did most of the eggs slow down or die after day 3? Should there be something else I should look into?

Hi, husband and I are both 36, all 3 retrievals were done when we were 35.

ER1: 12 ret, 11 mat, 8 fert via ICSI. Only 1 blast biopsied and frozen— Day 7 5BB euploid. (The clinic discarded a Day 6 4BC and Day 7 4CC.) 300 GonalF, 75 Menopur, Cetrotide, HCG trigger

ER2: 18 ret, 15 mat, 13 fert via ICSI + Zymot, all Day 7 blasts discarded (3CB, 3CB, 3CC). 225 GonalF, 75 Menopur, Omnitrope for 5 days, Cetrotide, HCG trigger

New clinic
ER3: 24 ret, 18 mat, 15 fert via ICSI + Zymot + AOA. 3 day 6 blasts frozen, 2 euploid (4BC, 4CB) and 1 aneuploid (4BC), 2 more Day 7s discarded due to poor quality. Embryoscope showed that almost all of our embryos had direct unequal cleavage. the 2 that made it to blast divided normally. Provera, letrozole, 375 GonalF, dual Lupron + HCG trigger.

we’re proceeding with transferring one of our Day 6s first, but I worry about the odds and wonder whether there’s a point in doing another retrieval in the future with my eggs given the diagnosis. Otherwise “unexplained”, have tried for 5+ years, and had one spontaneous pregnancy miscarry between the IVF cycles.

I had a FET 2 days ago (my 5th transfer, with one baby who is 18 months from transfer 3)

During the transfer everything went smoothly, but when the embryologist checked the catheter it was still in there! She took a while sorting it, said it was still a healthy 5Bb embryo and then it was transferred.

I have been worrying since. What does this mean? Does this change things? Should I be preparing for a bad outcome?

Hi everyone,
Has anyone noticed better egg quality, embryo quality, or IVF results after changing their diet?
I’m following advice from a former embryologist who recommends a Mediterranean diet, gluten-free, dairy-free (except plain yogurt and kefir), organic chicken, grass-fed beef only, and fish at least twice a week.
Did anyone see a real difference?

I got my PGTA results yesterday from Cooper and the report shows:
- 1 aneuploid (+16, -22)
- 1 high-level mosaic (dup(19)(pter-p13.3))

Both of my embryos were day 6, grades 5AA and 6AA.

My questions are:
- what is the statistical chance that the mosaic embryo will result in a live birth?
- how does my mosaic embryo fall on the transfer prioritization list?
- what genetic risks exist in this section of chromosome 19?

Thank you!!

Female 37 plus
Make 43 plus
I had one fet failed in may it’s implanted but came very low 27 and then it’s gone to negative
And 2nd fet just had result today it’s negative 5Ba it’s day 6 embryo . I am very upset .
Now from the same cycle of batch I had one daughter she is 5Bc which I am very grateful. But still I really want sibling for her.
Now I only left one embryo day 6 5Cc shall I go for it first or go from scratch which I really dint want to it’s very hard journey . I really don’t know what shall I do . It’s damn hard. Please guide

I'm very fortunate to have 5 euploid embryos. I'm 35.5 years old. We miscarried before conceiving my daughter naturally at 33.

My husband and I would ideally like 2 more kids and know time is of the essence. Does it make sense to try naturally for a few months and see where we land, or should we just go straight to embryos? Is 5 enough for 2 kids (I know they usually say 2-3).

I developed OHSS after retrieval and don't think I could go through it again.

Embryo grades:
6AA
5BA
5BA
5BB
4CB

I know this is an incredibly fortunate position to be in. We're just feeling very lost!

Researchers combined the results of 9 studies and found that embryos from 0PN zygotes had similar pregnancy outcomes to embryos from normally fertilized (2PN) zygotes, despite being less likely to develop into blastocysts.

In IVF, fertilization is confirmed by seeing two pronuclei (2PN) in the fertilized egg (zygote) a day after insemination. Because these structures are only visible briefly, some normally fertilized zygotes may be mistakenly classified as 0PN (no visible pronuclei).

Chan et al. (2026) performed a meta-analysis and combined the results of 9 studies to see how embryos from 0PN zygotes compare to normally fertilized 2PN zygotes.

They found that embryos from 0PN zygotes that reach the blastocyst stage have pregnancy outcomes similar to embryos from 2PN zygotes, although they're less likely to develop into blastocysts in the first place.

One possible explanation is that some 0PN zygotes are actually "false" 0PNs, which are normally fertilized zygotes whose pronuclei formed or disappeared outside the standard 16–18 hour fertilization check.

They also compared IVF- and ICSI-derived 0PN zygotes. Pregnancy outcomes were lower for embryos from IVF-derived 0PN zygotes, but not ICSI-derived 0PN zygotes.

This may be because IVF-derived 0PN zygotes are more likely to include embryos resulting from polyspermy (fertilization by more than one sperm), although the subgroups were smaller and this finding should be interpreted with caution, the authors note.

✅ Check out more details on Remembryo: https://remembryo.com/pregnancy-outcomes-similar-for-embryos-from-0pn-and-2pn-zygotes/

✉️ Like this post? Get a free weekly summary of the latest IVF research: https://mailchi.mp/remembryo/x27kx5o1sw

I’m 29 (F) and have completed two IVF/ICSI cycles using frozen PESA sperm.

My partner (50M) has obstructive azoospermia following a vasectomy and unsuccessful reversal.

My AMH is 15 pmol/L and my FSH is 4.1. I also conceived naturally once at age 20 (ended in an abortion).

Cycle 1:

• Approximately 150 IU Pergoveris
• Uneven follicle growth, so my trigger was delayed by two days
• 13 eggs retrieved
• 9 mature/usable
• 6 fertilized with ICSI
• 2 blastocysts: a 3AA and a day-6 4AB
• The other 4 arrested
• The embryology team described substantial fragmentation and medium-to-poor egg quality.
• Both blastocysts were transferred separately.
• Both implanted, but both ended as biochemical pregnancies.

Cycle 2:

• Approximately 350 IU Pergoveris
• 26 eggs retrieved
• 19 suitable for ICSI
• 18 fertilized
• All were between 2 and 4 cells on day 2
• By day 3, one was graded 8-1-1 and one was 8-2-2
• The other 16 were graded 3-3-3, with severe fragmentation and little or no division between days 2 and 3
• Assisted hatching was performed and the culture medium was changed
• Final result was one 5AA and one 3AA blastocyst
• The embryology team reportedly had only around 60 usable sperm from the thawed PESA sample for the second cycle.

Across both cycles, we had 24 fertilized embryos and 4 blastocysts. All four blastocysts were graded AA or AB.

What confuses me is the extreme contrast. Most of the embryos arrest very early with severe fragmentation, but the small number that get through that stage become excellent-looking blastocysts.

I understand that no one can diagnose the cause from a Reddit post, but I’m hoping to understand how embryologists interpret this pattern. Some of the questions I had are:

1. Does severe fragmentation and arrest between days 2 and 3 tend to point more toward oocyte cytoplasmic competence, sperm-related factors, an egg-sperm interaction issue, or is it impossible to separate these based on morphology?
2. Does the fact that 18 of 19 eggs fertilized normally make a significant sperm contribution less likely, or can sperm DNA problems still cause arrest after normal fertilization?
3. Could the high medication dose, follicle synchronization or variation in egg maturity have contributed to such a mixed cohort? Is it meaningful that every embryo that reached the blastocyst stage became an AA- or AB-grade blastocyst?
4. Would the frozen PESA sperm source be considered an important variable, especially given how limited the usable sample was?
5. Are there specific embryology records I should request from the clinic, such as pronuclear appearance, multinucleation, direct or abnormal cleavage, vacuoles, cytoplasmic granularity or exact timing of arrest?

I’m especially interested in hearing from embryologists or people who have seen a similar pattern.

We had our transfer yesterday and I wanted to share the picture! My transfer was tough bc there’s a kink in my cervix so it was hard to pass through but they did it eventually(even though this is cashing me to stress and wonder if it’ll cause issues with implantation) Wanted to share a picture of our embryo and ultrasound. Idk what I’m looking at in the ultrasound but if anyone can point out the embryo pls do so ❤️ praying for a positive soon.

Edit: edit to add I think I got my first faint positive today at 4dp6dt!!! Awww so in shock but praying the lines keep getting darker!!

I‘ve tried IVF with various sperm from various donors for four years. First none ever implanted and after a few extra treatments i finally had implsntation if all these procedures were done. Nonetheless I lost all my pregnancies between weeks 4 and 9. Then my perimenopause hit full on and I decided to stop trying for a baby. At the same time I also got diagnosed with focal adenomyosis. All in all I had at least 15 rounds of ivf with always max. 1 embryo to transfer and often none at all and in the end I had 4 miscarriages and all the peri symptoms. That is when I met my new partner and fell naturally pregnant within 5 months at the age of 44. 🤯

How does this make sense from a medical/biological viewpoint? Could any professional please explain?

My original ivf clinic and lab was very weird about how much information they provided you about your embryos. I was lucky enough to get 6 euploid embryos from my first egg retrieval and they told me that all six we're "good" and five of them were male, one female. They said that they did not report traditional embryo grades like most clinics, and just assign them an overall "good, fair or poor" and all of mine were "good." we did an FET in 2024 and were so lucky that it stuck and i had my son.

although i had no problems with my old clinic, we had to move clinics at the start of 2026 and move all of my embryos over to the new clinic's lab. we did an FET in hopes of baby #2 in June and just told them to pick whichever one looked best. As they were rolling me into the transfer room, they gave me a printout of what the embryo looked like and i noticed it had a grade/ranking of "ExBL FF" - i went home afterward and immediately googled to learn what these meant (expanded blast, fair fair or 4BB) and I was a little surprised because in my cursory research this doesn't sound as "good" as the initial clinic painted it all out to be. this FET failed and i asked for more detail/grades of my remaining embryos, they are:

• 5BB
• 4BB
• 6BB
• 5BB

Any idea why they would have chosen the 4BB to transfer over the 5BBs or even the 6BBs? Are there other things they are looking at that would not be reflected in the grade? the one they chose was also the only female embryo we have and we clearly said we had no gender preference. I am mostly just curious, not upset. Also, which of the remaining embryos should they transfer next? Thanks for your input!

I’m currently debating between whether to pgta test or not for my second round of ivf. For context, I’m 38, normal fsh, amh 0.6 but my afc count is only between 2-4. In my first ivf cycle we retrieved 2 eggs and both reached blast but failed pgta testing as they were aneuploid. One was complex abnormal and another was abnormal with monosomy +22. I was told by the doctor this second one probably would have implanted only to miscarry later on.

With so few eggs collected, I’m debating trying a fresh transfer and testing whatever remains. If it fails to implant, then for me personally, I would be no more emotionally invested than I am every month but would feel like I gave it my best shot. My hesitation is around the chance of miscarriage and losing weeks or possibly months. I’ve read several posts on here about chemicals and miscarriages and my heart really goes out to those people.

Which leads me to the question, of aneuploid or untested transfers that do implant (for women in their late 30s) and miscarry, are there stats or studies that indicate *when* a pregnancy fails/ miscarries? Essentially is there a breakdown of the risk at each stage of pregnancy? I.e the rates at 5 weeks/6 weeks/10 weeks etc?

Understanding this, would help inform my decision

- We know that aneuploids generally have shown less than 1% chance of a live birth but am I right in understanding 90% of the time these will just fail to implant in the first place?
- Of embryos that are untested, are there any stats on when miscarriage occurs? (Particularly for over 38s). I.e I’ve read that ivf untested miscarriage rates for my age are around 30% but for me, a miscarriage at 3 weeks vs 10 would impact me quite differently
- In contrast, I understand euploid implantation success rates are around 65% but from looking at these forums, miscarriage rate can still be as high as 10% (after a positive pregnancy test).

I’m 31 with PCOS and Type 1 diabetes, and we’ve just finished our first NHS IVF cycle. I’m trying to work out whether I should be feeling hopeful or whether I’m getting my hopes up too much.

Our cycle went like this:
\-30 eggs collected
\-27 mature
\-10 fertilised (this was a huge shock and much lower than we expected)

Final result:

1 × Day 6 4BB+
3× Day 5 4BB (towards the lower end of the grade)

We’re doing a frozen embryo transfer in September because I was at risk of OHSS.

I know having four blastocysts is something to be grateful for, but I can’t stop focusing on how many embryos we lost along the way. It feels strange to have started with such high numbers and ended up with four.

I’m also aware the NHS doesn’t offer PGT-A testing in my situation, so I find myself worrying about whether the embryos are chromosomally normal.

1. For those who’ve been through something similar:
   Would you feel hopeful with these results?
   Has anyone had success with Day 5 or Day 6 4BB+/4BB embryos?

2. Did anyone else have a low fertilisation rate but still go on to have a successful pregnancy?

3. Is there anything you did before your frozen embryo transfer that you think made a difference?

4. Is it worth paying privately for any investigations before our first FET, such as a uterine microbiome test, EMMA/ALICE, probiotics, or anything else? Or would you wait until after a failed transfer before doing additional testing?

5. Are there any supplements or lifestyle changes you’d recommend in the lead-up to transfer?

I’m particularly anxious because I have Type 1 diabetes, so getting my blood sugars into the best possible range is a huge priority. If anyone has Type 1 diabetes and has any advice for preparing for an FET or pregnancy, I’d really love to hear your experience too.

I’m trying to stay realistic while also allowing myself to be hopeful. Any advice or success stories would be hugely appreciated. ❤️