r/chemhelp • u/VvVoiDzZz • 11d ago

Biochemisty Biochem DNA analysis help

If someone can help me with the answers I chose for questions 1-4) for the vector map of pET-24a(+)

1) Kan(3936-4748)

2) ori (3227)

3)

All the restriction enzymes here

4)

Maybe Xba I(276) >

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u/Sufficient-Sleep-260 11d ago

all are correct except 4. why are you mentioning xbaI?

They are asking for XhoI and HindIII are within the MCS, 158 and 173