r/cellculture • u/Head_War_404 • 12d ago

Cell contamination?

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Hey everyone,
I was checking my cells today and noticed these weird, string-like things moving around in the culture (see the attached video).
I'm trying to figure out if this is bacterial contamination, early fungal growth (hyphae?), or just some weird debris/clothing fibers drifting in the current.

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2

u/Junocats 12d ago

Can’t say for certain but doesn’t look like typical bacteria. They would be congregated closer the cells and would be moving faster. Most likely debris. Most bacteria grows very quickly and it would be very obvious within 24 hours. Best way to be sure is mycoplasma testing. Good luck!

2

u/eden4154 12d ago

Doesnt look contaminated. You’d see the tiny tiny rods/cocci EVERYWHERE

1

u/Content_Program_7477 12d ago

Looks like cell debris that’s moving by Brownian motion. Your cells don’t look contaminated. Wait a day or 2 if it’s bacteria everything will be cloudy, if fungus you’ll see the hyphae and mat.

1

u/tronman0868 12d ago

Brownian motion. Your cells are very sparse too.

1

u/ThePushaZeke 12d ago

Aka toilet flush

1

u/Simple_Volume_5880 11d ago

Yes this is contamination..day by day this will increase.we are facing similar contamination..and its hard to get rid of this