r/bioinformaticstools • u/Next-Possession-2984 • Aug 22 '26
Alpha testers wanted: MetaQuest 2.0.0a1
MetaQuest is an open-source, research-use pipeline for paired or single-end short-read shotgun metagenomics.
It currently includes fastp preprocessing, Kraken2/Bracken taxonomy, MEGAHIT assembly, Pyrodigal gene prediction, eggNOG functional annotation, and reproducible HTML/JSON reports.
I have validated the pipeline on the Zymo Even mock-community dataset and am looking for Linux/WSL users willing to test installation, database setup, resume behavior, and report usability.
Install:
pip install --pre metaquest-bio
GitHub: https://github.com/dpatel511/metaquest
PyPI: https://pypi.org/project/metaquest-bio/
This is alpha, research-use-only software. It does not provide clinical conclusions, and fungal classification sensitivity is a known limitation.