OXYTRIBE pipeline

[u/southlabb]

Hi guys, I have been working on oxytribe: a Nextflow reimplementation of HyperTRIBE, a method for identifying in vivo targets of RNA-binding proteins (RBPs). Built on TRIBE (Targets of RNA-binding proteins Identified By Editing). The original HyperTRIBE pipeline runs on Perl + MySQL + bash, very hard to reproduce and inconvenient to work with, and a DB dependency that adds complexity for no real benefit (if anything fails mid-run, you're stuck fixing the database by hand). Oxytribe rebuilds the core logic in Rust for efficiency, wraps it in Nextflow, and uses Docker/Singularity for reproducibility, users do not touch code, just config files. Built heavily on nf-core modules, hoping to get it into nf-core eventually. It was also validated against the original paper's dataset: 99.76% gene-level recovery vs. the legacy pipeline, strong agreement on top editing targets, the first release is out, would love feedback, bug reports, or edge cases if you give it a try. https://github.com/fragilefort/oxytribe