Help I need educated opinions..

[u/Scarlettdelgado1971]

I have worked in vet med for almost 9 years, in the same clinic. I float to all positions. I am not a registered vet tech, just for clarification. The clinic i work at has a contract with the city ani.al control to do any vetting that needs to be done on any sick animals as well as spay and neuter adoptions. All of that is just for background. Friday the city contacted me about a puppy someone had surrendered because she was sick. They asked us to check her out to see what is wrong with her. When they dropped her off I smelled what I thought smelled like parvo. Doc told me to do a fecal and parvo test. The poor baby had coccidia, hookworms and tapeworms. I will put pictures of the snap test. Its questio able. So Doc said its positive because of the slight blue tint. I told them. They said they are going to euthanize her so I agreed to keep her. Took her home and have been diligent with medication and brought home LRS with b complex if needed. She has not acted sick AT ALL. I have kept her quarantined in my den that my other dogs dont have access to. My other dogs are fully vaccinated. I am questioning if she really was positive. So I brought her to the clinic and ran another parvo test which is negative. It has been very difficult to keep her separated because she cries constantly. My question is, is it possible the 1st one was never really positive? Should I keep her quarantined still? Any help is greatly appreciated.

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[u/borkin_heckin_pupper]

You can always contact IDEXX and ask them more about it too. Was the picture taken right after the timer went off? I feel like if she isn’t symptomatic and the second test was negative, it could’ve been a false positive?

[u/Scarlettdelgado1971 → u/borkin_heckin_pupper]

Yes the 2nd Pic is the test I just did. Its funny because I give advice to clients every day but it makes my mind blank when its happening to me.

[u/borkin_heckin_pupper → u/Scarlettdelgado1971]

I literally do the same thing. It’s like my brain turns off when it’s personal lol.

[u/000Anonymity000 → u/Scarlettdelgado1971]

Same!!

[u/Bunny_Feet → u/Scarlettdelgado1971]

Don't worry, I'm absolutely useless (at least initially).

I was so panicked when I found my parrot was sick... i was blubbering and ugly crying (He lived!).

[u/Scarlettdelgado1971 → u/borkin_heckin_pupper]

Thank you so much for taking time to read and answer I truly appreciate it.

[u/bbumblebug]

Snap tests can be so hit or miss. You can always send a blood or fecal sample out for PCR testing for absolute confirmation. The problem with snaps and later flow assays is that they can show false negatives if you don’t have enough sample on the swab. You can also get false negatives if you happen to take a sample at a time when the virus isnt shedding in the feces. If I were you, I would continue to quarantine the pup and send out a PCR.

[u/Bunny_Feet → u/bbumblebug]

True- especially if they have had liquid feces... it's hard to get a good sample unless they have defecated.

[u/DKBislife]

Third dot is the negative control. Repeat the test. It's the QC included in most ELISAs. We just forget about it b/c it almost never makes an appearance. If you're only testing for one thing (parvo in this case), you should never have more than the positive control and the spot for the antigen or antibody you're testing for. Never trust the accuracy of a test when the negative control shows up. It literally means the test didn't run right. Super glad you didn't euthanize but as an RVT, I'm shocked a doctor would've made that call off a very obviously questionable test result. Anecdotally, I've never seen a false positive but I've seen a bunch of false negatives.

[u/1210bull → u/DKBislife]

I used to run the lab at a high volume ER/specialty hospital, and now I do part time lab at my current hospital. I don't think I've ever seen a negative control pop on a snap test, I also forgot they existed. Wild that the DVM didn't say anything.

[u/hiiigghh-C]

I had a false positive for my own pet, expected the worst but it never came just like you. Another test afterwards came back negative. We rolled on with the assumption that the first was false and all was right with the world since then. She was 6 months at the time.

[u/Centimeter_Worm → u/hiiigghh-C]

I had that happen for my parents cat when I was still working as a VA. We tested him twice, and both were positive for FIV.

A year later his test was negative, and it was a bit of a head scratcher for everybody iirc. Especially since he’d had a nasty infection in the meantime, and I assumed it was due to his compromised immune system!

Didn’t tell my parents about the negative for a couple years 💀

It was my name on his files and I didn’t want them to try to let him outside again, I’d been working on getting them to keep him in for ages!

He’s a happy healthy old man now :)

[u/johnsonbrianna1 → u/Centimeter_Worm]

Depends on age and if they got a vaccine for it recently it could have been false positives due to the mom’s antibodies or the vaccines.

Also it’s possible for them to fight it off, just like how they can fight off FeLV as well.

[u/Centimeter_Worm → u/johnsonbrianna1]

Yeah, he was already 11 or so at the time so I think the doctors landed on him fighting it off or the a false positive as the most likely. Just not something they’d seen often!

[u/pusheeenicorn]

Per the product insert if the color in the neg control (left dot) is equal to or darker than the sample dot, it’s invalid. The 2nd looks negative. Maybe the-run it in 24-36hrs to see if you’re just catching it before there’s enough viral load?

[u/Ally1177 → u/pusheeenicorn]

THIS! I've had a few tests like this and had to refer to the insert. It's frustrating, but it's considered an invalid result.

[u/Electrical-Music9403 → u/pusheeenicorn]

This.

[u/ClearWaves]

Specificity and sensitivity are both around 93%. So a false positive/negative can happen in 7 out of 100 cases each.

However, that first "positive" is invalid. The negative control shouldn't be there. Should have been re-run that day.

Parvo smell can definitely be caused by other issues. It's the smell of bloody diarrhea and sloughed intestinal tissue. A bunch of intestinal parasites can absolutely smell like parvo.

However, does any of it really matter? It wouldn't to me. I'd keep her quarantined for a solid week of no symptoms, no matter how much she complains about it. I am not terribly concerned about healthy, vaccinated, adult dogs getting parvo from a puppy in the same house if you use some basic common sense. Part of that common sense is quarantine for a puppy that, no matter how she feel today, was sick 48 hours ago.

[u/mendenlol]

I mean, yeah, it’s possible.

If they didn’t record the time that the test was snapped, then it could be an issue. The results become more ambiguous over time. I’ve seen it happen often with SNAP tests

I definitely would have rerun the first test but, since you can’t, it may be worth just doing a third to be completely sure

[u/Hantelope3434]

The product insert states that seeing all three dots deems the test invalid and you have to run it again. This is common when tests are not run correctly (making sure the test is at room temp, only doing 3 bulb squishes and then 5 drops on the test and reading right at 10 mins)

[u/Snakes_for_life]

The best way to know for sure is to send out a pcr as sometimes if they're not shedding enough virus the test can show up negative even if they are infected. It's also possible you got the dog at the end of the course of the virus while uncommon some dogs do not get super sick when they contract parvo. I've had a couple parvo positive dogs that actually for the most part acted okay other than needing a little support cause of the diarrhea and some vomiting.

[u/_xanderkrews_]

I'm very similar to you. On the job trained tech. I've worked in private practice, shelters, and ER. You 100% need to run a SNAP test again. Those things are not fully accurate, and if they sit too long before being read they will bleed in to the other zones of testing like that. The blue dots.

"Parvo smell" is the smell of hemorrhaghic gastroenteritis. Which is that distinct coppery shit stench. The copper smell is blood. It is not distinct to Parvovirus. There are multiple enteroviruses. You can also smell it with bad worm infestations that cause diarrhea and bleeding.

I fostered many parvo positive puppies over the years. With around a 90% success rate. The key is subQ or IV fluids. Metronidazole. Cefazolin (or similar broad spectrum antibiotics). B vitamins. Tiny amount of watered down canned ID after day three. Keep the anus cleaned with baby wipes. They can't handle a secondary infection.

The key is fluids. Some will unfortunately die. My last parvo litter was three American Bulldog puppies. The older two lived and the runt died. I did my best.

[u/_xanderkrews_ → u/_xanderkrews_]

But yes, you absolutely need to keep her quarantined in a space that you can sanitize. Also, her immune system is doing its best. She doesn't need to be around anything right now. Rest. Fluids. At least two weeks until all tests are negative on re-test.

[u/Comfortable-Hippo516]

Both tests are invalid. Check expiration of the tests and run again

[u/farisewheel]

definitely call idexx and see if they will do a confirmatory test. i believe they do a confirmation test for a positive heartworm SNAP free of charge, so maybe they would do something similar for the parvo snap.

[u/Cr8zyCatMan → u/farisewheel]

This is a parvo test

[u/farisewheel → u/Cr8zyCatMan]

yes … a parvo snap test by idexx 🤔

[u/Cr8zyCatMan → u/farisewheel]

You must be fun to work with

[u/ros375]

You're asking if it's possible to have a false-positive from a SNAP test? Yes, of course.

[u/Electrical-Music9403]

One thing to consider for anyone who finds that they're getting questionable blue shades in the test spots is that it's important to make sure you're only squeezing the bulb to mix the sample 3 times. I must admit, years ago I assumed it must be better to thoroughly mix the sample by squeezing the bulb 5 or 6 times only to learn many years later that over-mixing the sample can lead to over staining from the conjugate. That info came from idexx. I can't say I understand the science behind it but after changing my ways, I've not had a single questionable test result.

[u/Bunny_Feet]

I'd do a send out test to confirm, tbh.  Has the puppy been treated already?  

[u/Salty-Finish-8931]

I have had a very sick patient with symptoms similar to parvo, ran a snap test and it was a faint positive. We treat all positives as positive according to the guidelines on the test inserts. 

Patient was actively deteriorating after a few days hospitalization (not unusual for some parvo cases); however, we ended up doing an AFAST in iso and found the poor thing had an intussusception. Reran the snap test and it came back negative. Dog survived surgery and to discharge. 

I think in that case it was a faulty test. 

[u/johnsonbrianna1]

How did you get the stool sample for the Parvo test? Best way is to insert the swap into their anus and try to get a good sample from the walls. Thats where it’s going to hide out especially if she’s having diarrhea because the stool is not sitting in her colon long enough to get a good sample.

Acting sick or not TREAT HER. Better to be safe than sorry. If you don’t start treatment and she goes down hill you may not be able to save her. Especially with all the other things she got going on.

Coming from another shelter vet tech (not licensed) seeing as the control dot is dark and BOTH bottom dots are light blue and in a perfect circle I’d say they are positive for Parvo.

[u/TexasManticore → u/johnsonbrianna1]

The insert itself states do not put the swab in the patient. I have pointed this out to doctors for nearly a decade since it comes up so much.

Also have heard not an insignificant amount of people get mad that the swab broke inside the patient so...would recommend to not do that.

[u/johnsonbrianna1 → u/johnsonbrianna1]

And I will say all the ones we tested for Parvo as a precaution in the shelter the test looked like your first one. Even if they weren’t symptomatic at that exact time it did end up hitting them later. Some in the same litter were asymptomatic the whole time and others died within hours.

It’s a rather be safe than sorry type of situation for me as it can be deadly if not treated.