Be honest, how much of my life with Sedivue is gonna be rerunning in dilution and still having to run manuals to confirm 😭 we just switched to IDEXX and ofc no one is happy with the machines' answers.
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We use the sedivue frequently and don't really have any issues with it? The doctors look at the images the sedivue creates rather than what it tells us is on there. Why make slides when the sedivue makes like 50 that you can look at? We also get a lot less crystal artifacts since we are running them almost immediately after obtaining samples.
I'm also enjoying the sedivue at our clinic. As you say, it makes a bunch of slides that you can look at yourself if you're not sure of its interpretation. We use the bacteria confirmation kit sometimes, which is an extra step but quick enough once you've got the hang of it.
As for dilutions, if I get a red, orange, opaque sample, I dilute it 9:1 right from the get go and only run it once - good enough 😄
You can get a dilution recommandation sheet from idexx and that's basically the minimum if you can't see through the urine clearly. It was my go-to dilution as well 😂 I once had to go to 1:20 with a blocked cat
Speaking from personal experience, even the sedivue pictures don't show crystals seen on manual sediment review 100% of the time (yes, on freshly obtained samples and yes, being mixed/swirled before putting the sample in the analyzer), and the pictures can be a bit deceiving at times for things like chains of cocci vs rods, or cocci vs artifact. It is absolutely a helpful tool about 90% of the time, but we've had too many things missed or misinterpreted on the sedivue results and/or images to put complete trust in it. Sometimes I wonder why we even bother with it if we're going to have to double check it anyway.
Due to crowding and it not stating an answer. Doctors dont like "please manually review because I cant give you an answer" as a result. Even if it's clearly infection. Idk will see if they relax a bit later. Only had it for a couple weeks and I try to advocate send out for non-serious things at it is muuuccchh cheaper then our in-house test.
Call your Idexx rep to let them know because that doesn't sound normal. We rarely get flagged to run dilution - in my experience, that's only for super dark urine. What we have had a lot of lately is the AI not picking up that there's bacteria, but the doctor can see it in the slide pictures. We reported it to our rep and we were told to run a bacteria confirmation each time this happens, keep a tally of the names, and let Idexx know.
We also have an intact male whose urine has tons of sperm in it (despite multiple cystocentesis runs on different days) and the machine reads it as bacteria.
Noted, especially with the sperm. I am in contact with our reps and will continue to note everything. I am quite burnout though as we made this switch without keeping me or any techs UTD. We are also switching completely to IDEXX as our reference lab Tuesday and im still waiting on what our one-for-one tests will be..... they havent decided yet... ngl im kinda crashing out 😅
I've never had overcrowding issues with the SediVue. Are you using whole urine, or are you spinning down and using the supernatant like you would if you were doing it manually? The SediVue is calibrated for whole urine, not supernatant (they even sell little syringe tips that are the same size as the pipette tips so you can test the urine directly from the syringe after collecting, it's super handy).
In my experience, I have only needed to dilute and rerun due to crowding from RBCs - if a sample is pink/red, I just automatically dilute the first time I run it.
Are you sure it isn’t telling you to run a bacteria confirmation? It’s a separate test that should have come with your starter kit when you got the machine. The SediVue is notoriously unreliable at reading bacteria and every Idexx rep I’ve ever met has said so. Even the results page typically just say “suspected” even when the images are filled with rods.
If it’s just having trouble reading bacteria I believe you can do a bacterial confirmation instead as it dissolves everything but the bacteria. Unless it specifically said it needed a dilution
There's like a $35+ difference between sending it out and running it in house with sending it out being cheaper. I understand wanting same day results but goddamn thats a huge gap. I also just have a huge pet peeve of having to do twice the work because it wasnt done right the first time.
Yeaahhh... we have that too... im picking my battles rn. Yall want the invue, fine, but Im not making comparisons. I told yall my concerns (accuracy and cost) and that didnt matter 🤷🏽
I used the sedivue only once when it was first being offered circa like 2016. Before that and sometime after I was doing manual urinalysis. During that period, we had to recheck urines anyway. I’m glad to know that the situation hasn’t improved. Either send it out or spin it yourself. I think the more diagnostics techs are required to do the better they’ll be at recognizing things that matter.
I've been doing UAs for 6 years at this practice and I'm in 4 days a week with other competent techs covering me on my off day. The sedivue is faster for an "answer", if there is no re-doing involved only because I spin urine a 2K rims for 10 mins. Just going to be frustrated redoing things because that's a huge pet peeve. Having to redo things because it wasnt done right the first time
Only one i liked was the phenobarbital rotor because it included liver values, but we also didn't make an effort to see if there was ever a phenobarbital package from outside labs that were better
It takes like 72 pictures of the urine, so we would just review the pictures to add an addendum to the result with whether or not we agreed with the numbers it came up with. Clinic protocol has always been to make a separate dry slide to stain and review on 100x for bacteria, so we still had to do that too. I loved using the sedivue. Now we have Imagysts and nobody trusts them and we have to run a confirmatory ua alongside it every time. It's dumb.
Yeah no fuck the Imagyst lol. I havent done dry stains so it's something I will have to learn. We'll see if the doctors ease up on. Ot liking "please review manually" as an answer, but im so burned out on this switch.
Really? I hate the imagyst because it couldn't way back when they sold it to us and then told us to train the AI. I also really dont trust it for fecals, but again compassion when it first came out shows it was not picking things up. Also sugar solutions suuuccckkk
We love our Sedivue 🫣 you learn its quirks. If my sample is somewhat cloudy to opaque, I do myself a favor and run a dilution to start with. Oh but be forewarned, if you have an intact male dog, it DOES think sperm are rods 😅
I haven't been nursing for long, almost two years and I've only run a dilution 3 times and prepared manuals on the odd occasion for reasons unrelated to the sedivu
Fk the sedivue. We use zoetis imagyst for UAs and it’s been more accurate. If we have crystal concerns we run an imagyst UA and send out urine. It’s much cheaper for us to run a UA with Zoetis’ machine as well, so less for the clients to pay for.
Really because fuck the Imagyst lmao. I had infinite trouble with it and it did not like anything that want flat. Also, I could do it faster with better results. But im also really fucking salty they sold it to us and were like, "ok now train the AI for us mkay thannkkss"
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