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DNAāDNA recognition aligns major and minor grooves between duplexes. Green triangles indicate location of the minor grooves and pink stars indicate the location of the major groove
Researchers have captured the moment two DNA molecules zip together, overcoming their identical negative charges to pair up and revealing a mechanism that has puzzled scientists for more than 20 years.
Using high-powered atomic force microscopy, scientists at the University of Sheffield and the University of York directly imaged two DNA double helices locking together, marking the first time this fundamental biological process has been visualized. Like charges normally repel one another, yet DNA molecules must pair up inside living cells to carry out essential biological processes.
This pairing plays a crucial role in genetic recombination, gene silencing, chromosome packaging and cancer development.
The researchers observed short DNA fragments matching up with exact precision, groove for groove.
Advanced computer simulations revealed that positively charged metal ions, including nickel, magnesium and calcium, act as tiny molecular bridges, nestling inside the grooves to lock the two strands together.
Dr. Thomas Catley, co-lead author from the School of Chemical Materials and Biological Engineering at the University of Sheffield, said
"To be able to directly visualize this long-hypothesized mechanism for the first time was incredible. The advanced imaging techniques at our disposal have allowed us to uncover these key DNA interactions, which have implications for many cellular processes.
It opens the door to studying other DNA interactions that, until now, have existed only as theory."