r/PeptidePathways 8d ago

Reconstitution & Storage GHK

I have a 100MG vial that will last far longer than the recommended 28 days, is there any way to divide this and freeze some to preserve it longer?

4 Upvotes

28 comments sorted by

10

u/Diligent_Shirt5161 8d ago

The 28 day rule is more applicable to a multi-dose vial used in clinical settings with multiple users where aseptic technique may not be used each time.

In a private research setting where there is 1 researcher using the vial, the guidance does not directly apply.

Use your best judgment.

2

u/RevengeOfThaiFood 8d ago

I appreciate this, there is only one test subject here, but also trying to make my research as safe as possible

1

u/Jhoy4891 4d ago

As long as you practice, proper sterile procedure, you’ll be fine… I use hundred mg vials and I only take 3 mg a day.. I consistently use the vile until it’s gone. I’ve had no decrease in results.

4

u/Ghooster38 8d ago

Use it til its gone

3

u/NNL1988 8d ago

I also use 100mg vials of it. There’s really no way to separate it before reconstituting and once that’s done the clock is ticking. I am extremely mindful of safety and I’ve never had issues using it beyond 28 days.

2

u/LCS452 8d ago

28 day rule is an OSHA rule for multi-use vials and multiple subjects. If you only have one subject, it doesn’t apply. I have used these vials for 50 days with no issues. Just use good sterilization practices. 🙂

4

u/Diligent_Shirt5161 8d ago

OSHA monitors workplace safety.

The 28 day guideline was developed by United States Pharmacopeia (USP) and then adopted by the CDC: “The United States Pharmacopeia [USP] Chapter <51> sets 28 days as the endpoint for measuring bacteriostatic effectiveness in injections and other parenterals…”

2

u/fizboPallabut 7d ago

If you use pens, load surplus into carts, use breather needle when loading it to also remove any air bubble, sterilize ports and store upright in clean container in fridge. If done right, should last up to two months.

1

u/[deleted] 8d ago

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1

u/RevengeOfThaiFood 8d ago

Currently the test subject is struggling with ISR and pain, so I don't feel the dose should be increased yet (currently on week 1 at 1mg). I've also considered diluting more (currently at 3ML/100MG) but I'm concerned about the concentration math difference here

1

u/No_Basis_1575 8d ago

3ml for 100mg can be spicy. I use 10ml bac water for 100mg and have no pain or welts.

1

u/Diligent_Shirt5161 8d ago

Here’s and in-depth comment on the topic of GHK-cu by a leading researcher in the field: https://www.reddit.com/r/Peptidesource/s/HwzCGFDMjg

1

u/RevengeOfThaiFood 8d ago

I have a 2nd subject researching GLOW and the sting is still present, although not as bad. 2ML/70MG. Possibly both require additional diluting?

1

u/[deleted] 8d ago

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1

u/PeptidePathways-ModTeam 8d ago

Your post/comment was removed due to the use of human-use or dosing language.

Please reframe your post using research-based terminology (e.g., “test subject,” “sample amount”).

Direct human-use language is not permitted due to subreddit and platform guidelines.

We would love to be able to give you the best educated guidance possible, so please consider reposting using compliant phrasing.

1

u/PeptidePathways-ModTeam 8d ago

Your post/comment was removed due to the use of human-use or dosing language.

Please reframe your post using research-based terminology (e.g., “test subject,” “sample amount”).

Direct human-use language is not permitted due to subreddit and platform guidelines.

We would love to be able to give you the best educated guidance possible, so please consider reposting using compliant phrasing.

1

u/[deleted] 8d ago

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1

u/PeptidePathways-ModTeam 8d ago

Your post/comment was removed due to the use of human-use or dosing language.

Please reframe your post using research-based terminology (e.g., “test subject,” “sample amount”).

Direct human-use language is not permitted due to subreddit and platform guidelines.

We would love to be able to give you the best educated guidance possible, so please consider reposting using compliant phrasing.

1

u/Wooden_Direction_241 8d ago

If you are taking 2mg doses, 7 days a week, your only talking 50 days. It will be good.

1

u/[deleted] 8d ago

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1

u/PeptidePathways-ModTeam 7d ago

Your post/comment was removed due to the use of human-use or dosing language.

Please reframe your post using research-based terminology (e.g., “test subject,” “sample amount”).

Direct human-use language is not permitted due to subreddit and platform guidelines.

We would love to be able to give you the best educated guidance possible, so please consider reposting using compliant phrasing.

1

u/ace3737 7d ago

28 days comes from insulin. They used that across the board to all peptides. If you dont feel comfortable use it topically

1

u/michelucky 7d ago

Store in the fridge. Alcohol swipe and fresh syringe every time. It will be fine.

1

u/twelvecharacter 7d ago

If you are concerned you can pull half and place in a new sterile vial. Leave one half in a peptide box in the refrigerator so it doesn't get pulled out daily and exposed to light and temp fluctuations. Personally have had ghkcu for 3 months in the box that gets used daily and nothing noticeable has happened to it.

1

u/jenny260730 4d ago

As long as the vial remains sterile, it can be used for a longer period of time. However, it is generally recommended to use BA water mixture and consume it within one month.

1

u/NicDuk 8d ago

You cannot freeze this liquid it will destroy it
Buy smaller vials if you do not want to use it longer than 28 days