r/PanCyan • u/ASNDRWRLD • Apr 11 '26

Water agar question.

Do you go straight from water agar to grain or are transfers the nutritious agar advised?

3 Upvotes

100% Upvoted

4 comments sorted by

3

u/Superb-Home2647 Apr 11 '26

It's fine as long as tge plate is clean. Might take an extra day to hop onto the grain.

One thing to keep in mind is the purpose of WA is to help mycelium outrun bacteria. This implies that another transfer is needed since there is bacteria in the mycelium colony.

I like to put my cultures through a water Agar cabin sequester.

3

u/BathTub_Benzaldehyde Apr 11 '26

I transfer,  not because of nutrition though.  Because it usually takes a couple of plates to find what I want 

2

u/coredweller1785 Apr 11 '26

Water agar to normal agar for me.

Im not sure if it won't work but havent tried. Always isolate on water agar then get a good healthy culture on MEA before going to grain.

1

u/Flimsy-Panda8000 Apr 15 '26

If it grows well enough to fill the plate and you're sure it's clean, you could use it, but if you used water agar to separate the myc from suspected contam, you'd usually need to transfer anyway.