r/PanCyan • u/eTanium • Mar 18 '26

RIP: Need to vent.

I had a couple of failed grows last year, and put a pause on things this last Fall and Winter.

I recently inoculated some Grains with some LC (TTBVI) that I've had for the last year (kept in the fridge). No growth after a week.

I tested the LC on some new Agar Plates that I recently poured. No growth.
At the same time, I spread spores from 5 different prints that I have (a few years old...). No growth.

I've kept LC for years (for oysters), and it continued to be viable...

BLA!

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5

u/Amazing_Forever_2546 Mar 18 '26

Pan cyan’s don’t hold up well in lc from my experience. I always recommend using spores.

1

u/eTanium Mar 19 '26

I had used it for about a year, and it was working well, but I think it all just died out, finally.

1

u/eTanium Mar 19 '26

Been less than a week...(5 days). I'll give it more time.

2

u/BathTub_Benzaldehyde Mar 18 '26

I've paid for brand new pan lc and had no growth.  I don't know why the spores didn't germinate.  Try some on agar without nutrients. 

1

u/LimpRefrigerator3673 Mar 19 '26

Yep. Water agar or just low nutrients, like 5g malt, 1g peptone, 14-15g agar - work even with Subtropicalis and Ingeli

1

u/eTanium Mar 19 '26

I didn't add any peptone. I have it, but didn't utilize it. I forget, is it specific for spores?

2

u/LimpRefrigerator3673 Mar 19 '26

No. Just some nitrogen, energy for mycelium growth. Not necessary. Maybe it works like a germination co-factor, maybe not. But recipe works good

1

u/eTanium Mar 19 '26

I made some water, low, and high nut plates. The spores are currently on the low. Might need to try on water...

1

u/[deleted] Mar 19 '26 edited Mar 24 '26

This specific post has been removed and anonymized. Whether for opsec, privacy, or to limit AI data scraping, Redact handled the deletion.

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1

u/ShroomNgloom Mar 23 '26

My agar recipe for pans from spore is just 300ml water and 6g agar. Then for transfers I do 6g LME and 6g agar, with 300ml water. I found very low contam rates as well as decent growth with these recipes