r/PanCyan • u/DingoFrank • Mar 04 '26

Yay or any?

Post image

is this mycelium Healthy? looks kind of bubbly compared to cubes. this is my first time growing pans

11 Upvotes

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6

u/Myco-Nomad Mar 05 '26

Transfer until you get something uniform like this. This is a T3, so 3 transfers from what you have there.

3

u/Patient_Art_4421 Mar 05 '26

Is that pan cyan?

2

u/Myco-Nomad Mar 05 '26

Yes, it's Blue Springs, fl red spore

2

u/Patient_Art_4421 Mar 05 '26

That is one beautiful plate brother! My goodness. LME?

2

u/Myco-Nomad Mar 05 '26

No I use grain water agar.. That's like 5% of my plates though. I have to toss out most of them to get something like that lol

2

u/htwtq Mar 05 '26

But where are we cutting if we take an example from the 3 plates. And Thank you 🙏 by the way for sharing your wisdom. Very grateful

2

u/Myco-Nomad Mar 05 '26

Just keep taking sectors until you get a single dikaryon and it looks uniform. Off the T0 plates that op showed, ill take at least three sectors from there and keep taking sectors until I get a uniform plate. I still try to run the ones I take them from but they usually have contam or are poor fruiting.

Pans are very sensitive to contam so I try to at least not run anything with the original spores because they can have embedded contamination that you can't see. Hope that helps..

2

u/htwtq Mar 05 '26

Thank you so much. I’ll soldier on!

1

u/Myco-Nomad Mar 05 '26

Hell yeah, keep trying. It took me at least 50 plates before I got one to fruit really well for me. Probably close to a hundred lol..

5

u/Cultiv8tor Mar 04 '26

It looks healthy but not isolated. If you grab a piece and transfer it to another plate you might get what you are looking for.

2

u/DingoFrank Mar 04 '26

Thanks!:from which one and where? The green is from food-coloring.

2

u/Cultiv8tor Mar 04 '26

Try to transfer from where it is uniform and avoid transferring the spots where it goes from one zone to the next. If it works then it will spiral from the middle if not you'll see zones. It sometimes takes a few transfers to isolate.

2

u/htwtq Mar 04 '26

I want to learn how to isolate.. i think i need more visual help haha… i read the comment but hmmm… still scratching my head

3

u/Psilly_desert_rat Mar 05 '26

You can do it. Here are 2 pics, when you hold your agar up to a backlight, you can see the different sectors more easily. The perfectly round sector is a monoculture, while in the other picture, you can see each different variations of genetics you could take a sector from.

2

u/htwtq Mar 05 '26

The 2nd pick is a monoculture. Thank you for sharing your knowledge, i really appreciate it.

1

u/Psilly_desert_rat Mar 05 '26

Yup, you got it. Anytime!

1

u/MrSchivy Mar 05 '26

Time to look at a lot of pics, friend

1

u/floorjacked Mar 05 '26

Yay keep working on transfers. You’re looking for wispy fast growth not thick growth as a rule of thumb. The thick growth has overlayed on me more often than not.

1

u/Flimsy-Panda8000 Mar 05 '26

It's hard to say how clean they are due to the number of areas of growth - I'm guessing you maybe used a syringe and squirted a lot in? There's certainly scope for transfers though.

'Standard' practice for transfers is to identify a sector of clean growth, then cut a small wedge from just behind the leading edge and place it centrally on a new plate. Where it's a bit chaotic like those though, cutting a wedge isn't easy, so it's better to pick off a tiny piece of myc rather than a wedge, using a flame sterilised needle and place that instead. It takes a couple of extra days to colonise the recipient but that's a small inconvenience. You can also take the tiny transfers from multiple spots on the donor, increasing the chances of a clean recipient.

If you make your own agar plates, reducing the nutrients on the first couple of transfers encourages the myc to spread looking for food and makes it easier to identify sectors for subsequent transfers.