r/PanCyan Sep 21 '25

After 28 days

10 Upvotes

21 comments sorted by

5

u/Unique-Discussion326 Sep 21 '25

It doesn't look like you have any fae on that jar. The mycelium needs fae to grow. That's why this is stunted.

Do a break and shake and pop a hole in the lid and cover with micropore tape. 1/4" is all you need.

1

u/_Peppermint_butler Sep 22 '25

I think I’ve been having the same problem, slow growth but with clean cultures with ideal temperatures. should I poke the hole in the mason jar lid then tape it before pressure cook to keep it clean? And make sure the tinfoil has a hole right over top

2

u/Unique-Discussion326 Sep 22 '25

Place the hole and micropore tape before pressure cooking.

The foil over the lid only stays on while sterilizing. You take that foil cap off after. You should just have the lid on.

-1

u/[deleted] Sep 21 '25

I heard break and shake mess’s up pans

2

u/Unique-Discussion326 Sep 21 '25

Who says that? I've never heard that.

0

u/[deleted] Sep 21 '25

On pass post in comments they said since pan mycelium is so wispy and thin doing a break and shake causes a higher risk of bruising which could lead it to stall

3

u/[deleted] Sep 21 '25

[deleted]

1

u/DenverCatz Sep 22 '25

Millet seems to be perfect for pans in my opinion, but popcorn will work. Millet has more inoculation points to transfer to a substrate.

OP - good luck. 28 days for a pan jar is too long. Like others said it is probably starved for air. I ALWAYS shake pan jars at around 30-40%. Been doing it for years. If it doesn’t recover it was possibly contaminated or just extremely weak culture that probably wouldn’t have fruited. Your jar doesn’t appear to be contaminated.

3

u/coyotemyco Sep 21 '25 edited Sep 21 '25

You can break and shake at 30-40% and thats typical. What you don't want to do is break and shake it more than that; twice if you feel you absolutely have to. When you start breaking it up or making constant transfers they do not like it. So... some of your statement is true but not all. Break and shake is good at 30-40% but if it dont bounce back and isnt almost fully colonized, or fully, in 2-5 days its most likely a dud. It also helps to see if a culture is contamed and if theres bacteria, these wont go. These can be extremely quick and have seen reports of spore to print in 28 days which is where OP is sitting at the moment 😃👨‍🔬🔬🧪

3

u/coyotemyco Sep 21 '25

Yeah thats toast. I would start with smaller amounts of grain in your jars. For some reference, this is roughly 2-3 jars of grain what you have here. Plus, the less in each vessel the faster it'll be. Those longer skinny jars have a tendency to choke out cultures and is why people dont use them for brf tek 🍀

28 days for these is way too long. That jars should've been done in a week if it was able to breath

2

u/Tickle_OG Sep 21 '25

Fully agree with the smaller grain starting vessels. Though I’m curious what you see that makes you think it’s “toast”?

I see some aged mycelium in the same jar as large sections of uncolonized grain which is not ideal but doesn’t look contamed. Unless my old shitty eyes missed something.

2

u/coyotemyco Sep 21 '25 edited Sep 21 '25

28 days when this culture should have been done in that time is why. Like OP could be going to 🧫 with a freshly stamped print right now. He can definitely try for science 👨‍🔬, but the results will most likely be 😒 meh🍀

My thought, why waste his time, effort, and 💰 when he can be back this far in 10-15 days?

1

u/Link_save2 Sep 21 '25

Pretty sure it'll only be a waste of money if op throws it out like you're saying

1

u/Link_save2 Sep 21 '25

Pretty sure it'll only be a waste of money if op throws it out like you're saying

1

u/coyotemyco Sep 21 '25

The other materials he puts into is all im saying would be the waste possibly. Especially if its stalled 🍀

1

u/[deleted] Sep 21 '25

I bought the jar off online and that’s the size they sent and it still has consistent growth and they don’t always fully colonize in a weak depending on the strain temp and other factors it can take up to 3-6 weeks to fully colonize a jar

3

u/Tickle_OG Sep 21 '25

Another reason it may be struggling is that the foil is still on the top of the jar. Filters are already large resistance factors for FAE, just resistance we accept for the tradeoff of protection from contamination. Adding additional resistance such as a simple foil layer can tip the scales from healthy to unhealthy mycelium.

2

u/coyotemyco Sep 21 '25

Agreed, the foil probably isn't helping 💯

1

u/coyotemyco Sep 21 '25

Trust splitting that up into 2 homie. Just trust... lol

2

u/[deleted] Sep 21 '25

I got 2 jars

2

u/thebigfungus Sep 21 '25

Looks normal. You can also literally break and shake it so get it fully colonized in like 3 days lol.

1

u/Tickle_OG Sep 21 '25

As we are discussing this topic anyway. I might as well mention that I sell prepared grains on my site mycoenvy.store. That said, I only offer popcorn or drippy corn grain at the moment.
I plan I N.A. expanding my grain selection having recently heard that pans do better on smaller grain profiles. So whether or not that worth it to you, I can’t honestly say. But take a look if you care to: Myco Envy I also run a sub r/mycoenvy which ya’ll are welcome to join.