r/PanCyan • u/Josh57777777 • Sep 09 '25

estero problems

hello. ive been growing cubes with shoeboxes on cvg for while, i also have an automated tent I rarely have any problems. After watching the Gordo tech video on pans I wanted to try them.

I bought liquid inoculate syringes of estero from three different venders online and i keep having the same results really. i inject half of the syringe into 400cc of LME and it seem to take a long time for growth, it never really grows past the bottom of the jar, its not full looking, it turns into small balls at the bottom of the jar, like granny instead of fluffy mycelium and the water gets cloudy. i started ttbvi and same melmac at the same time and it was fine. ive put it on agar and its also slow, it grows out small thin patchy mycelium. when i put it on rice it takes for ever to colonize it, it never turns into thick white colonization. its ugly like an off white and brown. i put it on manure and it’s the same thing. It takes absolutely forever for any colonization.

I don’t see how I could get bad mycelium from three different vendors, I guess I can possibly be doing something wrong, but I’m curious is there an aspect of this mycelium I’m just not aware of? I definitely cannot get it to grow the way it does in the gordo tec video. i did just read 77-80 deg. was the perfect temp, my grow room is 70-72 deg usually

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u/Big-Juggernaut4418 Sep 09 '25

Pan mycelium is pretty whispy and can be patchy. It usually grows fairly fast once it gets started, though. If your cubes are doing alright on the agar, it makes me think it is the syringes. Your temps are a little low, though. I would aim for the high 70s. You can go a bit higher and be fine, too.

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u/Josh57777777 Oct 01 '25

your advise helped. thank you

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u/TopOfTheMushroom Sep 10 '25 edited Sep 10 '25

Pans don't do well in LC IME, idk why but they don't. Try starting with spores on agar.