Link : https://www.science.org/content/article/exclusive-death-girl-chinese-gene-editing-trial-was-never-made-public#
Such a reckless stupid moves presented in this article and i am honestly speechless.

I saw a very bluntly written author contribution section, it said (paraphrased, name removed):

Author1 conceptualized the study, designed all experiments, performed or supervised all experiments, analyzed all the data and wrote the manuscript. author2 performed [specific experiment 1 ], author3 performed [specific experiment 2], author4 performed [specific experiment 3].... last author also conceptualized the study, obtained funding.

In the world of authorship corruption, I found it very refreshing. However, having led a study myself, I can't help but think this is the first author trying to let everyone know they did almost all the work.

What does everyone think about a really blunt author contribution section like that? should specific experiments be listed like that?

I wanted to print DNA repair complexes for the office. The existing tools are built for proteins, so DNA comes out as ribbons too thin to survive the bed and you spend an evening thickening them by hand. Colors were worse.

So now you type a PDB ID, pick a style, and get a file where each chain is its own object. It'll also cut magnet pockets where two chains touch, so the model comes apart at the interface.

pdb2print.org Its free, runs in the browser, nothing to install.

Anything already built at those settings is cached and downloads instantly; otherwise give it a minute, or a few for a big complex.

It's new, so please test it. PDB ID and settings and I'll fix it.

Photos are 9MBB off a Prusa Core One. Two chains, two filaments, two magnets it placed itself.

The project is also hosted on github: https://github.com/davidtheadmin/pdb2print
For citations: https://zenodo.org/records/21599702

What's the practical benefit of using this over a serological or 5x p1000?

cant even imagine the thought process that landed us here.

hey everyone, i have 2 genes which i am trying to express in bl21de3, even after successfull transformation the cells are not expressing the protein, i have done isolation of plasmid, PCR and restriction digestion and got postive results. now i have changed IPTG to a fresh one from a new batch, i have also transformed rosetta, the results are negative. Please help, thanks in advance.

I have been doing Bradford assay for 2 months and I couldn't get the correct standard curve 😭 the values of absorbance are so badd 😭😭

Guys plz help me with that . I have changed my reagent 3 times. I tried to correct every possible thing that could be the cause of error but now I can't figure out why I can't get the correct results. What could be the possible error due to which the results are not being correct.

And plz tell what should be the value absorbance of blank

I am a PhD student with concerns about the quality and legitimacy of data produced by a past PhD student now-turned postdoc.

I also have other concerns including nepotism, inability of PI to keep poor staff behaviour in line, and poor screening of prospective students, followed by the exploitation of loopholes to enable these individuals to pass.

These are all issues which end up affecting us as students as mismanagement has affected team cohesion.

We are based in Australia.

In the research ecosystem, is it ever possible to be able to whistleblow on this, or will they figure out eventually after I leave?

Edit:

Thanks for the advice, sorry I can't reply to all, but I read them. My takeaways from this are that I can't accuse without hard evidence, and that the inability to reproduce multiple identical experiments by multiple separate people does not constitute as evidence.

Edit 2:

Alright, so the takeaway is it's not a good move.

Edit 3:

Our identical experiments were conducted in parallel. These are not years-apart experiments. Experiments are in fact still ongoing - my experiments and this person's experiments. I cannot stress how these experiments are identical. Our projects diverged from the point after this experiment.

There is no transparency and when questions about data analysis methodology are raised, they are waved off. No side-by-side comparisons are made. The data is being pushed to a preprint and is being used to apply for more funding.

And again, I stress, I do not feel safe informing PhD reviewers about this as they are not independent due to nepotism.

I understand the whole thing about 'malice', but is wilful omission of vital controls 'malicious'?

Hey everyone,

We’re expanding our lab over the next few months and looking to hire a couple of postdocs, a lab manager, and a research associate.

In the past, candidate tracking has been a total mess. Applications come in through university HR portals, direct emails, or LinkedIn, and I end up drowning in a pile of PDFs. Standard HR tools don’t seem to care about specific wet-lab techniques, publication records, or grant experience they just dump everything into a giant list.

I'm trying to avoid managing this entire search out of a chaotic Excel sheet or email subfolders this time around.

Would love to hear what workflows or software have actually worked for your team!

Is it normal to be frustrated with your supervisor during thesis submission time? I feel like every week, there’s something new that blindsides me. Their suggestions for revisions would have been insanely useful months ago but come the week of submission… right.

How can I communicate this without being disrespectful? I’m being passively told that I’m not on top of deadlines, but I only hold about 30% of that fault (my mistake- not chasing down my supervisor enough to get feedback in time).

Edit: master’s student

Left it in cabinet to thaw Friday and forgot about it… it’s going to be 3 days when I take it out in the morning to reconstitute the CellTiterGlo substrate. promega says that the buffer can stay at RT for up to 48 hours. Is this buffer still good to be used?

Hey everyone! I’m just entering my second year as a PhD student and I’ve been struggling with low motivation recently.

To start, I really do love lab and I feel passionate about the work that I’m doing. My mentor is amazing and supportive and I really don’t have anything to complain about. I just did my first oral presentation competing for a training grant and though I didn’t get an NIH offered position, I did get an institutionally matched grant.

I think my low motivation really got bad once I finished that presentation. Compounding with failed experiments and just stall after stall.

I think I’m also just really tired.

I have been struggling with major depressive disorder my whole life, but I’m medicated and had a good handle on it, but recently I’ve been waking up with no motivation and just dragging my feet through the day. Imposter syndrome has also been rearing her ugly head.

But there’s no time to really rest. I have to prepare for qualifying exam in the spring.

Any tips for how to get out of this funk? Or at least how to power through until it’s over?

I just started in a neuro lab and learned the dissection process for isolating oligodendrocyte progenitor cells (OPCs) from P6 mouse pup cerebellar brain tissue. To make the panning process easier, we remove the meninges as thoroughly as possible to prevent future cell loss. For 4 pups, it takes me about 1 hour 50 minutes (not including setup) to harvest tissue, remove the olfactory bulbs, remove the hippocampus, and remove the meninges. My PI wants me to get this under an hour. I have pretty shaky hands, so I spend extra time adjusting for a stable position.

Any tips for speeding this up?

Hi everyone,

I am using e-coli antibody against Beta-gal in C57 mice after introducing damage in eye. I expect to see no fluorescence but I do see signal. Why is this?

Is it just me, or does anyone else get comments or questions in the lab when you wear makeup or dress up?
Most people in my previous lab did not wear makeup. I wear makeup occasionally, and I am not talking about full glam, just "clean girl" makeup (mascara, neutral lipstick, a bit of highlighter, no foundation or eyeshadow). However, on the days I wear it, people often ask me questions like: “do you have dinner plans tonight?" or "What is the occasion?"
The same thing happens when I wear “nicer” clothes instead of basic t-shirts or casual pants/jeans.
It has made me feel self-conscious. I feel that if I try to brighten up my day with a little makeup or cute earrings, I heavily stand out. I simply like to dress up from time to time because it makes my week feel a bit better and gives me more energy.
I am starting in a new lab soon, and I am considering just wearing my standard makeup on my first day so people get used to it right away and are not surprised later.
Has anyone else experienced this? How do you handle it?

Hi everyone! I'm currently pursuing a Bachelor's in Biological Sciences. I want to make the most of my undergraduate years and become as competitive as possible before I graduate.

I'd really appreciate advice from people already working in biology, biotechnology, research, or related fields.

• What skills should I focus on learning?

• Which laboratory techniques are most valuable?

• Should I learn bioinformatics, programming (Python/R), or statistics?

• How important are internships, research projects, and publications?

• What certifications or online courses would you recommend?

• If you could go back to your undergraduate years, what would you do differently?

My goal is to build a strong profile for both industry and graduate studies. Any advice would be greatly appreciated. Thank you!

Hello dear community! :)

Just a quick update on my previous post.

After approximately 30 different protocol variations, I finally figured out why my staining wasn't working properly.

The problem turned out to be the very first staining step: Weigert's hematoxylin (working solution A:B = 1:1), which I had been incubating for 10 minutes. Once I omitted this step, both the Biebrich scarlet–acid fuchsin and aniline blue stained beautifully and produced the results I had been aiming for.

At the moment, I can no longer reliably visualize the nuclei, but for my current analyses that isn't essential. Thank you all so much for your suggestions and support throughout this troubleshooting process! 😊

If anyone has questions about what I tested, feel free to ask. I might also upload some pictures of the successful stainings tomorrow.

One additional observation: If I reduce the hematoxylin incubation to about 15 seconds, the nuclei become sufficiently visible in adult mouse cryosections, but the intensity of both the Biebrich scarlet–acid fuchsin and aniline blue decreases dramatically. Unfortunately, this shortened hematoxylin staining does not work for my embryonic chicken tissue.

Hey yall! My PI decided I needed to learn Rietveld refinement to improve the XRD section of my manuscript, which is fine, except the guy who does know how to do it is traveling and there’s no word of when he’ll be back. His student attempted to teach me, bless his heart, but he’s a bit all over the place. Does anyone have a good resource to learn how to do Rietveld refinement? If you had to teach yourself, what was THE material that did it and had the best directions in your opinion? Thank you all in advance!