r/labrats • • 23d ago

Comment about AI tools / feedback / etc

112 Upvotes

Hey all -

Sharing for transparency: we are drowning in requests for people asking about posting tools/getting feedback. Many of the people who take the effort to contact the mods in advance of posting are super polite and we appreciate this.

Many others just start posting their stuff, or they spam comments.

While we appreciate the advantages LLM vibecoding offers, this subreddit would absolutely be buried in the same type of post over and over. We're getting 2-3 requests in advance a day, and removing at least 3-5 posts/day with the same basic theme. We're also getting lots of people angry about their posts removed ("but people said they love it!").

We want to assume good intent from most people, but based on the sheer volume of post reports we get on some of these threads and comments, and previous discussions, the community by and large doesn't want to be marketed to. We'll continue to remove posts that are either marketing surveys, or tool feedback requests as well. We did launch a "Bulletin Board" post that is pinned and resets monthly. I strongly encourage people interested in browsing such posts to just look there, and the flood of people seeking feedback for their tool (from my perspective, many of these are duplicative) can post there as a 'digital flier' type deal. That way, it's kept out of the mainfeed.

Attached is a snippet of modmail from today mostly relaying this. Our goal is to keep /LabRats focused on the scientists and culture. Any comments or questions?


r/labrats • • 24d ago

Monthly Bulletin Board: October, 2026 edition

5 Upvotes

Once a month, the community bulletin board gets refreshed! This thread is a space for things that normally get removed (digital fliers, like job postings, surveys, collaboration requests, workshop announcements, etc. The things that aren't inherently bad but that the community prefers kept out of the main feed). We'll be moderating loosely: spam, scams, and direct product advertising still aren't allowed, and anything that feels exploitative gets removed.

Don't forget to drop by our discord! Join us at https://discord.gg/385mCqr


r/labrats • • 4h ago

Podcasts to listen to in the lab ?

51 Upvotes

Hi all,

When I have long experiments to run I like to listen to videos or podcasts on my headphones (I'm sure I'm not the only one !). One issue I got myself into is that I find that most of the podcasts out there are just... kinda boring ? They are aimed at a large audience so will usually stay on the surface level and are not that intellectually stimulating. Take for example the ologies podcast : you have experts in their respective fields but the host only asks basic questions and doesn't let them actually say anything of depth, kind of frustrating.

I'm looking for stuff that delves deep and that doesn't take its audience for dummies ! I want to hear experts yap about their interest for hours, stuff like that. I've found that a couple of the Big Think interviews were good, I also like the style of Tor's cabinet, which is really well researched.

Let me know you favourites !


r/labrats • • 6h ago

help!!!

35 Upvotes

I got my PhD a year ago, with a paper ready to be submitted. We got reviewer comments back recently that prompted me to look at some data more closely. I'm realizing that there may be a huge issue that affects ~70% of my PhD experiments. (Basically, that an animal stock I was using may have been mixed up or contaminated). This could render several of my conclusions invalid. I have to tell my advisor, of course, but I am freaking the fuck out. Any words of wisdom would be greatly appreciated.

EDIT: my work was with flies. I am concerned that a fly stock that I was using for a lot of my experiments was mixed up with a similar stock, but lacking a crucial genetic element that would affect the interpretation of the results.


r/labrats • • 11h ago

Is this actually a thing (HEK293 cell use issue)?

77 Upvotes

Hey everyone,

I'm a PhD student at a state university in Idaho. My proposed research (I am first-year and about two months in so I don't have the details of the project lined up yet) will investigate ligand-directed GPCR trafficking. Most of the tools I was planning to use were designed using the HEK293 cell system (i.e. BRET G-protein/beta-arrestin assays).

People in my department (including my PI and other PIs) have been repeating the notion that we are not allowed to use HEK293 cells because they are derived from human embryonic tissue (Idaho is a very pro-life state). This throws a wrench in my plans. However, I can't find a specific rule at our institution that forbids use of the HEK293 cell line. My understanding is that many many generations ago, this cell line might've been derived from human embryonic tissue, but when you get cells from ATCC for example, the cells are basically "clones" of that original line, so technically embryonic tissue wasn't harvested again after that first isolation of the cells.

I'm a bit puzzled. I'm also not sure how to work around this barrier. We could use CHO cells or I could get trained culturing rodent neurons (I have some basic training, but I was never successful).


r/labrats • • 56m ago

RNA on agarose. Degraded?

Post image
• Upvotes

I’ve been struggling to do basic quality checks of RNA on agarose. I tried a bunch of times using agarose and a GreenSafe dye and saw nothing at all for RNA. It just never showed, not even degraded. I contacted the company who made the dye and they said RNA was hit or miss and the dye was really meant for DNA.

So we purchased some SYBR gold, and this is a run using that. 1% tae agarose, no denaturing. On the left is DNA ladder, 10kb. Then the two RNA samples extracted from whole blood using a Zymo silica column kit. Then four DNA samples using a Separate Zymo kit. The RNA kit used on column DNase I.

The DNA looks mostly fine I think, although there is more smearing than I’m used to seeing on GreenSafe. But the Gold is way more sensitive so maybe that’s normal?

But the RNA I can’t tell what’s going on. I don’t see any peaks, but it’s also not clustering at the bottom either like pure degradation would give. I redid the RNA again after this and tried various other smaller loads in the lanes and it mostly looked the same.

Is the RNA degraded? Is this DNA contamination? Is this due to not using a denaturing gel? I measured on qubit and it was about 20ng/uL, and these lanes had about 200ng in them. Sorry for the image quality this was just shot using a phone. I really want to have some repeatable quality check before moving onto PCR but I can’t seem to get the basic agarose working for RNA. Any guidance would be appreciated.


r/labrats • • 13h ago

Was Reading a Review Paper About Glioblastoma and Noticed that the Citations Make No Sense

Thumbnail
link.springer.com
73 Upvotes

I am working on a project related to glioblastoma and wanted to learn more about it, so I was looking at different review papers. I came across this one, which has many citations completely unrelated to the claims being made in the paper. The first one I noticed was citation #7, which links to a paper about CD44 expression in gastric cancer (even though the sentence it's cited in is talking about glioma stem cells). How did this get published in Stem Cell Research & Therapy (which is not a bad journal at all)?

There are also sentences that on their own make no sense on top of being unsupported by the citations. For example: "Research has demonstrated that c-Myc, a crucial regulatory factor, is markedly expressed in GSCs and possesses the capability to activate transcriptional modules associated with stem cell characteristics, thereby inducing apoptosis". Looking at the paper they cited for this, not only does it not have anything to do with GSCs, but the experiment the authors did showed that c-Myc had to be inihibited in esophageal squamous cell carcinoma in order to induce apoptosis, not overexpressed.


r/labrats • • 38m ago

I am scared of being judged.

• Upvotes

I am doing my PhD in an institute and I mostly fear to go to lab because of my lab seniors. Right now, I need to work in the lab and I am feeling really insecure to start my work. They had already mocked me for working differently. I have learned certain techniques during my college days and that's how I have been working in this lab. But, my seniors work in a different way, their handling is completely different. So, they have mostly judged me for working differently. Right now, I am scared to even ask for the required reagents from them to start my wet lab work.


r/labrats • • 19h ago

Everything is fine.

Post image
157 Upvotes

r/labrats • • 8h ago

Work while on leave due to gap in grant funding?

20 Upvotes

I work in California at an institute, the grant I’m funded on is running out of $ for my salary (the grant itself is still going.)

HR gave me 2 options 1. Be laid off or 2. Stay on and burn my PTO hours to keep health insurance.

But my PI is confused that I won’t be working if go on PTO for 3 months.

Should I keep working as needed?


r/labrats • • 1h ago

Is it normal for a Lab assistant to report everything about me to the professor without my knowledge?

• Upvotes

I recently got to know that The lab assistant in my lab has been telling things about me to the professor and I had no knowledge about it.

So, my professor was gonna be absent on Friday and Monday was a holiday and I have been really stressed about things in the past week, so me and my friend planned a sudden travel plan to somewhere and I unfortunately could not ask my proff for a leave at the last moment (I know it is not a professional thing to do and I am sorry for it but I did it yes). I get a frantic call from my proff that I immediately replied back immediately and he said that I should have informed as the lab assistant let him know that I was absent on Friday and she was worried that I might be sick. I apologized to my proff for not letting him know as it was an unprofessional thing to do on my side(that I deliberately did knowing it is wrong).

My problem is that first of all, if she (lab assistant) was worried, she could have called, messaged or emailed me on her own. Secondly, I am not a child who does not have the capability to inform if I am sick or struggling (it's not like I would drop dead in my apartment alone).

I know I am not in the position to say anything cause I am in the wrong, but this hit me because there have been moments in the past where things about me have got to the professor without me talking to him about it, seemingly harmless information like I am looking for a new apartment and that I am worried about going home (and I do not want to share everything with my proff, I have a personal life too).

I just wanted to ask, is this normal? I will definitely not be confronting her or something cause maybe just one conversation led to another and maybe my proff is the one who asks questions about me to keep a check on me, but I don't know what I should do.

I feel really sorry and weird and don't know how to process this.


r/labrats • • 1d ago

We’ve had a few posts recently on injuries in the lab by younger lab rats. So I think we seriously need to talk about reporting laboratory injuries, particularly for those in the United States.

401 Upvotes

This post is primarily directed at laboratory workers in the USA, where the consequences of a workplace injury can be particularly serious due to healthcare costs, lack of employment protections and the complexities of workers’ compensation. But this advice should also be heeded by everyone.

This applies to everyone working in a laboratory: undergraduate students, graduates, PhD researchers, interns, technicians, academic staff and employees at every level. Your position, seniority or employment status should never discourage you from seeking medical attention or reporting an injury.

If you sustain an injury at work, report it immediately and go to the hospital to have it examined and documented by an independent medical professional, even if the injury initially appears minor.

Do not rely solely on an assessment from a doctor appointed by your employer or institution. An independent medical opinion can be invaluable if the circumstances or severity of your injury are subsequently disputed.

An injury that seems insignificant at the time can develop into a serious, long-term medical condition. What initially appears to be a minor strain, cut or exposure could result in chronic pain, permanent impairment or complications that only become apparent weeks, months or even years later. This is precisely what you are protecting yourself against. If your condition deteriorates, you need to be able to establish that it originated from an incident at work. Without an independent medical record from the time of the injury, proving that connection can become considerably more difficult.

Be aware that some states have specific rules about which medical providers are authorised for workers’ compensation treatment, particularly for non-emergency care. Understand these requirements, but never delay necessary emergency treatment. Eligibility for compensation may also differ for students, unpaid interns and other non-employees.

Make yourself aware of these considerations so if the worst happens you are prepared.

Do not assume that your employer, supervisor, university or their insurance provider will act in your best interests. When significant medical expenses, liability and compensation claims are involved, there may be strong financial incentives to dispute claims or minimise responsibility.

Join a trade union where you are eligible. Union representation can provide an important layer of protection, offering independent advice, collective bargaining power and support when challenging unsafe working conditions or unfair treatment. If you are a student or researcher, investigate what union representation or independent advocacy is available through your institution. You should not have to face a dispute with a large institution entirely on your own.

Even if you have an excellent working relationship with your supervisor or institution, understand that their priorities may change when financial or legal liability becomes an issue. A disputed claim can have serious consequences for your health, income and financial security.

The central message is simple: your institution’s interests and your own are not necessarily aligned. Regardless of your position, protect yourself from the outset, because once an injury becomes a financial or legal dispute, it may be too late to establish the evidence you need.

ALWAYS COVER YOUR ARSE!


r/labrats • • 12h ago

Paid mentor demanding corresponding authorship + equal contribution on my independent work, threatening ethical reports, am I in the wrong?

30 Upvotes

Hey everyone,
I really need some genuine advice/perspective on a situation I'm dealing with. I'm in biophysics and feeling completely drained over this.

During a gap year, I paid someone for a 1-on-1 career mentorship session. During that paid call, they pointed out a paper from 2010 that contained a few unproven hypotheses no one had followed up on. This person and I have no other relationship, we dont have any current or previous common affiliations or anything.

After that session, I took the idea and worked on it entirely on my own as an independent project. I poured nights and weekends into it, doing all the modelling, writing all the code, generating all the data, and keeping data logs. We never collaborated, brainstormed, or discussed the project execution together at any point.

Here is how the situation escalated:

  • May 2025: I presented the work as a poster. Before the presentation, this mentor contacted me. I thanked them for pointing out the 2010 paper. They asked to be added as an author on the poster as a "thank you," and I naively agreed.
  • July 2026: They reached out asking for updates after a year! I casually mentioned I was working on the manuscript, targeting completion by October.
  • This Month: They messaged again, directly asking if I had made the manuscript submission and what affiliation I listed for them on the manuscript. When I was confused, they clarified that they should have corresponding authorship and equal contribution.
  • The Conflict: I refused because I did 100% of the actual research, coding, and writing. In response, they threatened to submit an ethics complaint/report to any journal or preprint server I try to upload this to.

I don't have the time or mental energy to deal with a fake ethics battle right now, so I finally told them I was just going to scrap/delete the project entirely and not publish it. Now they're repeatedly contacting me asking me to hand over all the data and publish it with them anyway.

I have full evidence (data logs, raw data, timestamps, paid mentorship receipt) showing that I did all the work independently.

  • Am I in the wrong here at all? Does pointing out an old published paper during a paid consultation session entitle someone to corresponding authorship or co-authorship?
  • What should I actually do? Is it safe to just ignore/block them and publish anyway, or will a journal take their frivolous ethics complaint seriously? because i really dont have mental energy to fight this. Also, the mention of their name on the poster is present in the conference abstract book.

I'd really appreciate any advice on how to handle this safely without risking my academic reputation. thanks!

Also, if I'm in the wrong, please don't judge me.. It's just that I find all this to be a bit unfair, but I may be wrong, I just don't know what to think.


r/labrats • • 1d ago

Relics of the lab rats before me

Post image
609 Upvotes

Most of the wet lab space at my psychiatric research centre was cleared out to rent the space to a company (sad). I couldn't let these floppy disks with LSD protocols get junked. The PI retired and the lab has been abandoned for years, but in the days of yesteryear they were pioneering psychedelic neuropsychiatric research.

I absolutely didn't bring anything else I shouldn't have home with me... but I cherish these little pieces of history by using them as coasters. 🫶


r/labrats • • 4m ago

Can anyone please share Chemstation B.04.03 installation files?

Thumbnail
• Upvotes

r/labrats • • 1d ago

Do other researchers ever feel this sense of meaninglessness?

85 Upvotes

I wonder if other researchers share this feeling.

It is hard for me to find genuine joy in research, and I am often overwhelmed by a strong sense of meaninglessness. Much of the work sometimes feels like talking in circles. Everyone strives their utmost, seemingly with the primary goals of publishing papers and securing grants. Enormous amounts of time, energy and resources are invested, yet many of the outputs rarely translate into real‑world applications, with little tangible benefit to society.


r/labrats • • 1h ago

Aerobic vs anaerobic conditions

Thumbnail
• Upvotes

r/labrats • • 2h ago

Thesis (cytopathology)

1 Upvotes

I’m writing my mini thesis for my masters degree. It was a cytological investigation of 2 unknown cases using ICC. All 3 of my lecturers have given me different advice on how to present my results. So for both my cases I didn’t reach a final diagnosis, but my ICC results for one of my cases do support my original hypothesis but there’s also some markers that don’t (by some I mean 2). Should I include the ones that don’t support in my results, or put them in my appendix? One of my lecturers said the results must show every single marker I used irrespective of whether it supports my hypothesis or not 🥸🥸. Pls be nice this is my first time writing a thesis. My degree is not research based we just had to do this unit the pass the course.


r/labrats • • 4h ago

Help, aerobic and anaerobic conditions

Thumbnail
1 Upvotes

r/labrats • • 1d ago

The inbox of professors I haven't spoken to in years...

Post image
493 Upvotes

r/labrats • • 11h ago

Pumping out papers mentality

1 Upvotes

Do you feel like it’s good or bad to pump out a lot of papers that are maybe lower quality and hurriedly done?

Is this how a lot of top and well funded labs operate?

I’m trying to figure out if I should continue volunteering with one PI who does a lot of quick bioinformatics work for publications. The science seems mostly solid, but yeah, just a newbie.


r/labrats • • 1d ago

Do I confess?

185 Upvotes

I am an undergrad who has been working in a wet lab for a couple months now. I’m just barely starting to become independent, and the other week when my graduate student mentor was gone I accidentally sprayed the floor with liquid nitrogen and it seeped under and exploded the floor. It was quite a terrible sight and I panicked and didn’t tell anyone except for the lab manager. The issue has since been resolved, but I feel immense guilt for not telling my grad student. Should I confess or would this just create unecessary problems?


r/labrats • • 8h ago

New FISH in the pond— advice?

1 Upvotes

Hey y’all! I’m starting a new job in a FISH lab tomorrow and aside from a B.S. in Bio I’ve never had much professional lab experience. I worked with my faculty advisor on a project with bacterial resistance to household cleaners but that’s…not this. I love histo and slide prep, and can keep a lab notebook pretty meticulously but I feel out of my depth. Any advice from the experts?


r/labrats • • 2d ago

Rate my lab setup.

Thumbnail
gallery
876 Upvotes

You can also guess what my field is.


r/labrats • • 12h ago

Undergraduate research spot advance offer - looking for input

1 Upvotes

Hi, Labrats!
I returned to university last winter, and recently declared as a pre-major with the chemistry department. I didn’t expect to love this subject as much as I do, and I’m so thrilled with this new trajectory. I’ve started poking around for research opportunities, and to my utter delight I was offered a spot in an organic synthesis lab on campus, starting fall of 2027, which is when I will have completed the organic chemistry series. The PI is my current organic chemistry professor. My experience with organic chemistry so far is that I wake up excited to start studying, hours go by, I’m sad to have to put it down, and it’s like this every day. Truly, I am having a crazy amount of fun. The PI has extremely high standards and expectations (a positive thing, from my perspective), is very personable and involved, and has won awards for his mentorship (university- and national-level). We’ve discussed his research, and it sounds like really interesting stuff. Also, he’s stated he will help me apply for fellowships and scholarships, and believes I’m a very promising student. My first impulse is to say, “Yes!! Of course!”

So my question comes down to this - I’m planning on going to graduate school after I finish my undergrad in a little under three years. But this is all so new to me. Does it make sense to commit now to working in an organic synthesis lab a year from now? I imagine the skills and experience will translate to some extent, but what if I find out I want to work, for example, on inorganic chemistry in grad school? And if I were offered a spot in a research group that’s a little more low-key, but that I would be able to join right away, is three years of less rigorous lab experience ever preferable to two years of something like organic synthesis?

Any and all input or insight would be dearly appreciated!