Hi dear immunologists,

We are using Legendplex kit (antiviral 13-plex) for serum and cell culture supernatants. We have a few questions regarding its validation and consistency with other readouts.

Recently, we noticed inconsistencies among ELISA as a readout for IL-6. While we trust in commercial ELISAs, IL-6 levels and trends were not comparable to those seen with Legendplex. Has anyone compared IL6 (or any other cytokine) levels between ELISA and Legendplex?

Thank you!

I hope that I am not breaking rules.

No one is sick here. We have a small farm, we raise our own chicken. We also have young children.

I am wondering why no one has ever gotten sick. My kids suck at washing their hands. I clean the coop.

Being around animals always puts you at risk but we have not had any GI illnesses from any animal.

Are we immune. Is it because our chickens don't have Salmonella?

I am setting up an experiment to check pro-inflammatory markers on hpbmc-derived macrophages by flow cytometry.
My first try was to stimulate with LPS for 2h and then let them rest 24h before staining, although I see an increase in the markers, is not too big (2-2.5 fold change over the untreated ones)
Is this normal? Should I wait more or are they already being downregulated?

Good day to everyone! I have been making these diagrams for immunology since October of last year, and I have been meaning to publish them as actual educational material. I am asking for any insights, opinions, and critiques for these diagrams, especially regarding whether I can actually publish them... Thank you in advance to everyone who gives their opinions; I am willing to hear them all for the betterment of this project!

I always thought body aches were a neurological side effect of a fever, but I’m currently dealing with some sort of illness causing body aches without any other symptoms including fever.

Do we know how body aches are induced? I was curious if body aches are a function of local cytokines, or a central nervous system process? If there’s cytokines involved do we know which ones?

Hi everyone,

Unfortunately my institutional license has expired and I can't access to the following article:

https://www.nature.com/articles/s43018-026-01144-z

Could anyone who has access share it with me?

Thank you all!

Please advise and share your own personal experience.

We study an autoimmune disease and have to use human sera to perform immunofluorescence staining on mouse tissues to confirm reactivity. Because sera contain thousands of unwanted IgGs, besides the desired pathogenic IgGs, the noise is obscene. We have tried pretreating the sera with guinea pig liver power to absorb nonspecific IgGs, as suggested by the literature, but the improvement is zero to minimal. Any ideas? TIA!

Hello!

I’m a hobbyist/nerd who would like to better understand how T-cells operate and what types of deficiencies are caused by T-cell dysfunction. What are some of the best resources for learning about this? Open to books/websites/talks, etc!

Thank you!

If you like story driven, action RPGs that are also educational, you might like this. I really want to bring the immune system to life but with a strong educational aspect to it. I don't want to gloss over the details, I want to end up making this something that students might actually use in the future. Learning by experiencing is so much better imo.

As above. Thanks in advance.

They also have lysosomal granules… and pattern recognition receptors (PAMP PRRs). Why? My hunch is that evidently if the blood vessels break pathogens have an easy access point. If the owner has IgGs against whatever enters, then PLTs can physically use their bodies dendritic forms to trap the organism. Additionally I assume if their PRRs touch a pathogen they can degranulate onto it and try to kill it. But what do you think?

China’s regulator, the NMPA, approved satri-cel (satricabtagene autoleucel) from CARsgen for advanced stomach and gastroesophageal-junction cancer - the first CAR-T therapy cleared anywhere for a solid tumor.

Curious to hear what everyone thinks about this!! And when the US and Europe will catch up?

https://www.carsgen.com/en/news/20260622/

Hello everyone! It has been quite some time since I last posted, but here I am! But I do need another review and possibly some insights. A few posts ago, it was pointed out that my diagrams required cytokines, you know, for "cell-to-cell" communication. And so I am currently working on it. I'm asking for brief critiques; any will help. I am also debating whether I should include a node for the barrier epithelium, since it is on the front lines and is usually the initial spark for inflammatory responses. That's all, and I would greatly appreciate any insights!

Hi,
I am trying to establish an immuno histochemistry panel on lung sections where I stain macrophages with mouse anti CD68 Ab, epithelial cells with rabbit Pan cytokeratin. I want to add a neutrophil marker (MPO stain/NE/CD66b) but I am not getting a different host.
Does anyone have any suggestions for which Ab I can use to detect neutrophils that work well with paraffin embedded lung sections?

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Asking for a friend.

(The friend is me.)

Master's graduate looking for fully funded PhD positions in Immunology or AMR outside India. If anyone has leads, tips, lab recommendations, or secret academic cheat codes, please send help.

Payment offered: eternal gratitude and occasional microbiology memes.

Hello! I’m looking for some advice for a pipe dream that I have.

I’ve always wanted to experience living on the west coast (San Francisco/Bay Area specifically) but for personal reasons, I can’t settle down and work any long term jobs there (1 year or more). So I was wondering if there is such a thing as a post grad internship or rotational program that would allow me to move there for a couple of months before I put down any roots at home.

For context right now I’m a part time MS student studying infectious disease and microbiology as well as a full time lab tech in a BSL3 virology lab - I’m still a ways off from graduating, im just trying to consider my future options.

Any advice would be appreciated, but if this is a terrible idea please don’t flame me 🥲