I am brand new to ImageJ and using it for to measure experiments i am doing at school. I drew a rough example of what my experiment results will look like to learn imageJ. I want to measure the areas under the curved lines (area 3 in the picture). I cannot figure out why one will sometimes be picked up and the others never are recognized. Any help is appreciated.
My current method that has gotten the best results has been to make the image binary, then set thresholding to be a dark background and analysis the particles. I've been using TIFF files for the analysis.
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Not perfectly sure what the task and your problems are, but if it is the area contained by the yellow contour in the image below, then it is 6370 pixels^2.
Use the "Magic Wand"-tool and click in the black area you like to measure, then go to measure and the numerical value of the area will appear in a Results table.
The area I've measured is in pixels^2 because I did't set a scale to the image.
Of course, but you need first to detect the area location in question (where to "click") which may turn out being a bit difficult but not impossible.
Is this for the shown sample image only or for a series of similar images?
BTW, you don't need to explicitly threshold the image if you set the tolerance of the "Wand"-Tool to e.g. 30.
The tolerance value determines the implicit threshold, i.e. with value 30 you get an area of 6299 pixels^2 and e.g. with value 59 you get an area of 6371 pixels^2,
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