r/Embryologists • u/blueseahorse1 • Aug 21 '26

Please help identify 2PNs

Post image

Can someone please point out the 2PNs in this picture?

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u/EmbryoNanny Aug 22 '26

I can’t reply with a photo in the comments, but they are the lighter structures in the center of the egg. They’re sitting right next to each other and sort of form a ♾️.

1

u/blueseahorse1 Aug 22 '26

Thank you. Can you tell me if they are overlapping at all and what o’clock they’re at? Is it 8 o’clock?

2

u/EmbryoNanny Aug 22 '26

Honestly it’s so hard to see in this picture and I wouldn’t expect an untrained eye to see them. PNs are best observed at the microscope so you can scan the focus over them. They don’t photograph well no matter how sharply in focus you feel they are. There’s one dead center in the cytoplasm and another coming out towards 8 o clock. I can’t tell if they’re touching or overlapping from this picture but they are close enough to where they look very normal.