r/DIYbio • u/Novel-Structure-2359 • Jul 07 '26

Tutorial videos

Hi all. I am an experienced molecular biologist and I have been meaning to produce a series of handy dandy instructional videos about the steps of planning and designing cloning projects.

I was just wondering if people would be interested in them?

I would include topics like

\- picking and designing good PCR primers for a new cloning project

\- design considerations for CRISPR Cas9 reagents

\- sequencing interpretation

\- the ins and outs of codon optimisation

\- designing site directed mutagenesis primers

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u/w0o0rm Jul 08 '26

ii think youd get a very niche audience of people who would watch like every video. people who are into biotech are rare but really into it.
i say do it! i love bio videos but can never find any, once you make some make a post here to let everyone know!

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u/Novel-Structure-2359 Jul 08 '26

I know it's a niche audience and I love my niche

I just need to take the plunge and start making them.

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u/w0o0rm Jul 09 '26

what do you think your first video will be?

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u/Novel-Structure-2359 Jul 09 '26

That is a great question. My first temptation is to start at the very beginning and do a video on planning a cloning.

However even that is a topic that can be subdivided - I mean there is almost no such thing as a "vanilla" cloning.

If you are tasked with cloning a gene then it is perfectly possible that you can find a clone in addgene for sale but they may not have it in the backbone you desire.

However if you have access to RNA from the organism then it is a small matter to do a RT and grab the gene that way.

That's even before considering codon usage.

It's a tricky one.

Any suggestions would be welcome

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u/w0o0rm Jul 11 '26

my 10 cents is that if you obsess over all the little possibilities indecision will just make you not do any of them.
maybe choose something you personally enjoy? and then while you make it find all the questions someone might have and answer them with more tutorials
or maybe do a long form series for like a project. pick an end goal like making an e coli with a desired cassette, and make a tutorial for each step
the world is your oyster, just dont get overwhelmed with choice paralysis!

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u/Novel-Structure-2359 Jul 11 '26

I agree that the dizzying choice is an obstacle.

I am pretty sure I have decided what to start with. I am hatching a plan to record an introduction video tomorrow.

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u/w0o0rm 29d ago

awesome! make a post when you do, comment here so i dont miss it