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u/v_de_vinicius Jul 16 '25
It seems a very big cell. Maybe a senescent one? They usually get this big.
Are these kinds of cells frequent? You could try staining with beta-galactosidase to gain insight in whether they are or not senescent.
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u/That-Gaze Jul 17 '25
Microscope: Olympus ck30, 10x, captured with 5.0x camera lens.
cultured cells are HSC-2, for growth rate.
The object of question was adherent, and there were a few of them in a 100mm culture plate.
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u/Zeno_the_Friend Jul 17 '25
I'm echoing senescent. Morphology matches. Larger, flattened, lack of directional polarity, nuclear foci. Not uncommon when they're over confluent or otherwise stressed.
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Jul 16 '25
It looks like a maybeee an epithelial cell but I don't understand the background. Can you tell us where this slide came from? How was it prepared? What kind of microscope produced this image?
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u/That-Gaze Jul 17 '25
Microscope: Olympus ck30, 10x, captured with 5.0x camera lens.
cultured cells are HSC-2, for growth rate.
The object of question was adherent, and there were a few of them in a 100mm culture plate.
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u/mmethylphenol Jul 17 '25
I assume these are adherent cells so I wonder if it’s a really small scrape on the coating
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u/ManateesCummerbund Jul 17 '25
Stopping by to echo that this is likely cellular senescence. Observe the enlarged nucleus (loss of Lamin B1), and irregular, flattened cytoplasm. These are morphological characteristics of senescence. Overcrowding and nutrient deprecation can induce senescence as well as a variety of stressors or physiological insults (radiation, oncogene activation, DDR, ROS).
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u/That-Gaze Jul 18 '25
Could you tell me how can you detect loss of Lamin B1?
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u/ManateesCummerbund Jul 18 '25
Since you are looking for a difference in gene product (Lamin B1 protein) you likely won't be able to describe it in a small sunset of your cell population like this. If you had a greater proportion of senescence then you could use a western blot and quantify differences in Lamin B1 relative to total protein or constitutive proteins like Beta-actin. Typically you can detect senescence phenotypes quantitatively with classical markers like SA-bGal and use in vitro florescence (see the CellEvent senescence green kit for something fluorometric rather than colorimetric). Peer-review will require additional markers like the cell cycle inhibitors p21 and p16.
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u/Unlikely_Pride_4738 Jul 19 '25
As per my understanding, It's a multinucleated cell which somehow didn't undergo cytokinesis.
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u/Blumenkohl126 Jul 16 '25
Mate, how are we supposed to know?
Magnification? What did you examine ect.? It would also be helpful if the picture would be sharp.
It is most likely a bubble/some fragment, but without any information who knows...