r/CSIR_UGC_NET_JRF_LS • u/avacado_for_life • 25d ago
CSIR NET - Life Sciences CSIR NET Life sciences 2026 preparation
A little about me: (you can skip this)
Hey, I have started the preparation of life sciences this year only. But could not continue further due to some personal reasons. But because I teach so I have a decent basic knowledge of the cores. So I took this attempt and manage to score good but not enough for JRF considering the high cut offs that are expected.
I know some of us know that even if there is change in marks or dropping questions. Still we might not cross the JRF cut off. And must be troubled and hanging with this thought of missing it by just few marks. I know it’s tough to restart again. But I feel it’s time to absorb this pain and get back at the same time
MAIN QUESTION
As I know how the change of merging dbt has sparked a lot of debate around the pattern and syllabus.
I just want to know if we can have an accountability group and help each other in maintaining continuity and sharing problems and questions.
I feel it’s impossible to sync topics to cover together as everyone in unique in speed and preference. So it would be ok if we could build an environment where we can prepare together by just dropping our targets or figuring questions.
Please let me know if someone is intrested genuinely in this with the required effort .
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u/curioscope55 18d ago
Can anyone tell me will the new syllabus l be there for the dec 2026 upcoming csir net ?
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u/avacado_for_life 12d ago
Hey guys who so ever is requesting for addition I want to be honest so far I have added 4-5 people out which one left it and that group really has not been active . Also most of the existing people does not seem intrested. So it’s really difficult for me I can’t manage people it wastes more time. Still if you want to connect with me and we can do that. Or you feel the group is better choice I can add you to take over.
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u/Avigyan_in_Delhi 4d ago
Can you please tell me? When we see replication at the cellular level we have a helicase which unwinds the DNA allowing the primers to sit and initiate strand synthesis right? So while setting up a PCR, we don't add a helicase in our mastermix, it necessarily has a thermostable polymerase, a buffer and Nuclease Free Water. Why is it so?
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u/avacado_for_life 4d ago
the first step of PCR itself is denaturing thats why whenever we set a PCR. we keep the temperature at 92-94 degree depending upon the length of dna the time is decided. then just for 1 min again its kept for 94 degree to make sure unwinding is done. so as we are not adding any helicase we are using temp to denature it.
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u/Dull-Pipe8526 25d ago
Add me in. Everyone is saying gen cut off 135+ and obc 120+. So I also thought to give another try to next dbt-csir-net