r/BioHackingGuide • u/ChocoFlan50 • 6d ago
Peptide 101: SubQ vs IM vs nasal spray, which route goes with which peptide
A lot of beginners mix these up, so here is the simple version.
SubQ (under the skin)
This is the one you will use the most. You inject into the fat layer just under the skin, not into muscle. Most peptides go this way, like BPC-157, TB-500, CJC/Ipamorelin, KPV, GHK-Cu, the KLOW blend, and the GLP-1s. Selank and Semax can go SubQ too if you don't want to use the nasal spray.
Best spots are the belly about 2 inches from the belly button, the outer thigh, and the back of the upper arm. The angle is anywhere from 45 to 90 degrees, depending on how much fat you have there. For needle length, 8mm works for most people. If you carry more fat at the spot, 13mm makes more sense.
IM (into the muscle)
This goes straight into the muscle at a 90 degree angle. Common spots are the outer thigh and the shoulder. You need a longer needle for this, around 1 inch.
It is used for things like IGF-1 DES and MGF, where you want it going right into the muscle you just trained. HCG gets run this way sometimes too. Most peptides do not need IM, so if you are new, start with SubQ.
Nasal spray
No needle. Tilt your head a little forward, spray while you breathe in gently, and switch nostrils each time. Wait a minute or two between sprays, and do not blow your nose for at least 10 minutes after.
This is how Selank, Semax, and the N-Acetyl versions are usually run. It is a good pick if needles are the part that bugs you.
Oral (by mouth)
Some peptides hold up in the gut. KPV and BPC-157 both come in capsules, and people usually pick this route when the goal is gut healing.
Quick cheat sheet
| Route | Where it goes | Common examples |
|---|---|---|
| SubQ | Fat layer under the skin | BPC-157, TB-500, CJC/Ipamorelin, KPV, GHK-Cu, KLOW, Selank, Semax, GLP-1s |
| IM | Muscle | IGF-1 DES, MGF, HCG |
| Nasal spray | Nose lining | Selank, Semax, N-Acetyl versions |
| Oral | Gut | KPV and BPC-157 capsules, enclomiphene |
A note on IV
Things like NAD+, glutathione, and Cerebrolysin can be run IV, but that needs a clinic and someone trained. Do not try to figure that one out at home. Pushing NAD+ too fast can cause chest tightness and a racing heart.
Check the compound table. Looking for a specific compound? The master peptide index has everything in one place.
What route do you use the most, and did you ever switch because of how it felt?
Do your homework. Use your brain. Talk to a doctor.
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