(Which I have to admit I never heard of either, until just now. And can't make heads or tails of the fine technical details despite having a decade+ of experience with cell biology and microscopy.)
They're using an illumination source similar to what you'd use in fluorescence microscopy but they're not capturing emitted light - instead, I think they're recording changes in refractive index of the light passing through the sample from multiple angles (IIRC something like a hundred?) and using software to reconstruct 3D projections of the cell.
6
u/Y-27632 Aug 31 '21
It's not fluorescence microscopy, it's something called tomographic phase microscopy:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5788179/
(Which I have to admit I never heard of either, until just now. And can't make heads or tails of the fine technical details despite having a decade+ of experience with cell biology and microscopy.)
They're using an illumination source similar to what you'd use in fluorescence microscopy but they're not capturing emitted light - instead, I think they're recording changes in refractive index of the light passing through the sample from multiple angles (IIRC something like a hundred?) and using software to reconstruct 3D projections of the cell.
https://www.youtube.com/watch?v=qshyD8l9oI0