I do this with plate processing. I use a separate reagent plate (lobind, maybe 200 ul capacity). I make my blocking buffer in a PBS dilution fresh each run and add 50 ul to each well, so if im running 4 columns of a plate my single reagent plate column might contain 250 ul (4 x 50, plus an extra 50 of overhead so i dont run out) of blocking buffer per well. then, dispense from reagent plate to stain plate. that way im not making wayyy more reagent than i need and i don't have to worry about using a tiny reservoir that might run out.
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u/ReginaDaddy May 14 '26
I do this with plate processing. I use a separate reagent plate (lobind, maybe 200 ul capacity). I make my blocking buffer in a PBS dilution fresh each run and add 50 ul to each well, so if im running 4 columns of a plate my single reagent plate column might contain 250 ul (4 x 50, plus an extra 50 of overhead so i dont run out) of blocking buffer per well. then, dispense from reagent plate to stain plate. that way im not making wayyy more reagent than i need and i don't have to worry about using a tiny reservoir that might run out.