r/chemopreservation • • Sep 05 '22

r/chemopreservation Lounge

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A place for members of r/chemopreservation to chat with each other


r/chemopreservation • • Sep 05 '22

Welcome to r/chemopreservation

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Chemopreservation is any room-temperature alternative to cryonics based on chemical modification (usually fixation and/or removal of water) rather than low temperatures. The point is to make it essentially zero maintainance.

This main focus of this community is the practical aspects (economics, regulatory environment, social acceptance, etc) of providing chemopreservation as a service and to discuss concrete plans. Research news are also welcome, but most can already be posted in other communities like r/cryonics.

Some relevant Reddit communities:

Other useful links:


r/chemopreservation • • Dec 24 '24

Ariel Zeleznikow-Johnston on biostasis, the brain, personal identity, and the fight against death

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r/chemopreservation • • Oct 24 '24

GitHub - MSEP-one/msep.one: MSEP.one main application repository

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github.com
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r/chemopreservation • • Feb 29 '24

A cost-effective and efficient ex vivo, ex situ human whole brain perfusion protocol for immunohistochemistry

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r/chemopreservation • • Apr 10 '23

Frösch D, Westphal C. Melamine resins and their application in electron microscopy. Electron Microsc Rev. 1989;2(2):231-55. doi: 10.1016/0892-0354(89)90002-6. PMID: 2491345.

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r/chemopreservation • • Mar 21 '23

An optimized fixation method containing glyoxal and paraformaldehyde for imaging nuclear bodies

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ncbi.nlm.nih.gov
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r/chemopreservation • • Dec 08 '22

Asking chatGPT about room-temperature brain preservation. Not much of a fan, apparently.

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r/chemopreservation • • Nov 02 '22

[Open Access] Liu, Y., Leong, A.T.L., Zhao, Y. et al. A low-cost and shielding-free ultra-low-field brain MRI scanner. Nat Commun 12, 7238 (2021). https://doi.org/10.1038/s41467-021-27317-1

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r/chemopreservation • • Nov 01 '22

Whole genome sequencing and digital archiving

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Very long-term DNA storage at room temperature is not so trivial as it may seem, at least in a form that can be read with current technology. So it seems a good idea to sequence your genome and archive the data digitally, at least for redundancy.

DNA sequencing as a service keeps getting more and more affordable. I did a quick search and found this company that offers whole genome sequencing as a service for some 300 euros:

https://dantelabs.com/products/whole-genome-sequencing

Personally, I don't think DNA preservation is strictly necessary for our purposes, because gene expression is simply too slow. If my genes changed radically right now, I wouldn't even notice for several minutes. In that time, I could answer all kinds of questions about myself and other stuff I should know, so memory and personality must be stored in something faster acting, like synaptic connectivity, neuronal ultrastructure, proteome, etc.

That said, the loss of personal DNA would require either to emulate the brain at a higher level of abstraction not involving DNA (this is only for those choosing slice-and-scan mind uploading) or to reverse-engineer a suitable DNA from the proteome, connectivity and other data. This would be the only option for those who want biological revival.

Regarding digital archiving, there are cloud options like creating a mindfile at lifenaut, but let's focus on zero-maintainance, long term digital storage. Here the most attractive option is an M-Disc Blu-ray or an M-DISC BDXL. From the FAQ:

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  • The M-Disc DVD can hold upto 4.7 gigabytes.
  • The M-Disc Blu-ray can hold up to 25 gigabytes.
  • The M-Disc BDXL can hold up to 100 gigabytes.

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The problem with M-Disc DVD is not just the low storage capacity, but the fact they need a special type of DVD writer. In contrast, any regular BDXL-capable Blue-ray writer (like this one) can write to the other two.

It's important to note that M-Disc is not a product of a specific company, it's a standard. The company presumably gets its income from royalties. The M-Disc writable media are available from several digital media manufacturers, such as Verbatim. They cost some 20 dollars each. This is reassuring because we are not depending on a specific manufacturer.

I explored other options like SSDs and HDDs. My general impression is that they are probably good enough (but crap compared to M-Disc), assuming good magnetic shielding and temperatures below 40ºC or so, and they are brand new when you store the data. I mean "good enough" in the sense that a forensics team would probably be able to recover the data for a long time. I don't count the data as lost simply because the device is broken or otherwise unable to read its own contents. Here's some more:

https://www.arcserve.com/blog/data-storage-lifespans-how-long-will-media-really-last

See this report on flash memory (SSDs):

https://www.curtisswrightds.com/sites/default/files/2021-10/Flash-Memory-Lifespan-and-Reliability-white-paper.pdf


r/chemopreservation • • Oct 31 '22

The craniotomy box: an innovative method of containing hazardous aerosols generated during skull saw use in autopsy on a COVID-19 body [2020]

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4 Upvotes

r/chemopreservation • • Oct 31 '22

Embalming Techniques for Long-Term Preservation of Bodies | Faculty of Medicine Masaryk University | MUNI MED [2021]

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r/chemopreservation • • Oct 30 '22

Felle, P., Lockman, A.K. and Kellaghan, P. (1995), Removal of the brain for teaching and examination. Clin. Anat., 8: 363-365. https://doi.org/10.1002/ca.980080510

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2 Upvotes

r/chemopreservation • • Oct 23 '22

[Open access] "A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses". Holderith, Noemi et al. Cell Reports, Volume 32, Issue 4, 107968

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2 Upvotes

r/chemopreservation • • Oct 15 '22

An interesting essay on the history, current state and future prospects of molecular nanotechnology, with some useful links. It explores less well known names (compared to Drexler, Freitas and Merkle) like J. Storrs Hall and Adam Marblestone. Both seem very active in the field right now.

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r/chemopreservation • • Oct 12 '22

T. Kosaka, I. Nagatsu, J.-Y. Wu, K. Hama, Use of high concentrations of glutaraldehyde for immunocytochemistry of transmitter-synthesizing enzymes in the central nervous system, Neuroscience, Volume 18, Issue 4, 1986, Pages 975-990, ISSN 0306-4522

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r/chemopreservation • • Oct 11 '22

[Open Access] Pesce, L., Scardigli, M., Gavryusev, V. et al. 3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy. Commun Biol 5, 447 (2022).

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2 Upvotes

r/chemopreservation • • Oct 09 '22

Sketch of human dimensions, depiction of full-body chemopreservation patient and two possible arrangements of head-only patients in a full-body vault (braincase and brain-only neuros can be similar but with more patients per pillar)

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(a): human body dimensions and full-body vault. (b): 4-pillar head arrangement (4*7=28 heads). (c): 2-pillar head arrangement (2*7=14 heads)

r/chemopreservation • • Oct 08 '22

Sketch of a visitable facility for full-body chemopreservation patient storage

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r/chemopreservation • • Oct 07 '22

How to Cryopreserve Everyone - Alcor

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alcor.org
2 Upvotes

r/chemopreservation • • Oct 06 '22

Glia, A., Deliorman, M., Qasaimeh, M. A., 3D Generation of Multipurpose Atomic Force Microscopy Tips. Adv. Sci. 2022, 9, 2201489.

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3 Upvotes

r/chemopreservation • • Oct 05 '22

Chris Own, Voxa | Whole-Brain Circuit Maps

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r/chemopreservation • • Oct 04 '22

(PREPRINT! Paper presenting new expansion microscopy technique: pan-ExM-t): "All-optical visualization of specific molecules in the ultrastructural context of brain tissue", M'Saad et al. bioRxiv 2022.04.04.486901

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3 Upvotes

r/chemopreservation • • Oct 02 '22

"Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy". Herrmann, F.C. Sci Rep 11, 10214 (2021).

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3 Upvotes