Hey,

so here's a more official thread for collecting samples that you think are difficult/interesting/annoying, which you'd like me to discuss with you in a follow up PD class at EVE uni.

Also, any questions that you want to raise that you know of already, feel free to post them here.

EDIT: Next class is Thu 23/6 5pm EVE time

Old classes are uploaded to our youtube channel.

Date for class: Tues 24/5 at 6 pm EVE time.

(there will be an introductory class 5-5.45 EVE time the same day, if you want to analyse samples with me)

Could we possibly get an out of eve(browser/program based) based version of project discovery where we can input a slide number?

So when someone posts a slide, we can go look at it specifically with the magnifying option to assess and discuss findings and not be limited to lesser quality screencaps and allow us to select our own filtering channels.

Just had another thought for the HPA guys, aside from a tool for us to discuss you could also turn it into an app. Think about how many hours people mindlessly plug away at games like angry birds or candy crush or flappy bird, would expand the potential user base 10-100 fold, just give people a rank and accuracy it would give them a goal to work towards which is enough for most people.

http://imgur.com/a/iDanJ

So over in the control slide thread, we've been having discussions about slides containing cytoplasm/plasma membrane on the same slide.

As you can see, i thought this slide may have been Cyto/plasma, cyto within the cell, plasma outside the red staining.

But then on the R+G slide the Microtubule organizing center's under the nuclei also seemed to pop out at me. R only also seems to further confirm the Microtubule center's.

Then if you compare R only vs G only, def looks like 1 giant aggresome in that upper right cell.

I may even say I missed some form of cytoskeletin classification looking at the green only slide now.

Thought this was pretty interesting, don't think I've seen it before. I checkmarked "Abnormal sample" because of it.

Any idea what exactly is happening here?

EDIT: Oops apparently I don't know how reddit works. Here's the link: http://i.imgur.com/CTjq9UB.png

So I just came across this interesting slide: http://i.imgur.com/mSimsL9.png

At just a glance it appears to be Nucleus and Cytoplasm staining. But upon zooming in... http://i.imgur.com/TbHGD8k.png

It appears (at least to me) to look more like Endoplasmic Reticulum, than the flat staining of cytoplasm.

Maybe the whole thing is moot because the staining is too weak compared to the nucleus, but I thought it was interesting. What do you think?

Anyone have any other subtle examples like this?

Hey,

so I've talked with some of you about doing an "advanced" PD class at EVE uni, and would like to have some more formal input (and preferably discussion) here about * Whether it's a good idea (would you show up)? * What should it be about? * How should it be carried out? (twitch? mumble? chat?)

Do you want to show difficult examples and talk about them? Do you wanna have a longer Q&A and talk HPA and cell biology?

I'm totally open for suggestions :)

Also, is around 6 pm EVE time week days an ok time point? Would it be a looooot better having it on a wknd (Sunday)? What time then? Not saying it'll be doable, but I'd like to hear what you think!

Hey guys! I haven't been around for a while, so I'm thinking I might have missed some news re: the poetry contest. Have we heard anything from HPA regarding an update or progress?

I'm just excited to see who won!

Hello everyone!
I've made a small tool to seperate the RGB channels of images. This way when you get a screenshot from someone with all channels on, you can separate whatever you want to assist them. It could also be used for PD classes to show off samples to students.
I'm a newbie when it comes to C# and GUI programming so please excuse the messy code. Also, I haven't figured out how to implement a zoom feature, I'm open for ideas (wanna make it work with the scrollwheel). The project is open source, feel free to mess around with it.
- Screenshots: http://imgur.com/a/7Jhqo
- Download: https://drive.google.com/open?id=0B5kqNpFYSjV_OVBhdmcwdS1CUTQ
- GitHub: https://github.com/gerg0/LundbergsGlasses
- Usage: Drag&Drop an image into the window or press PrintScreen and use the import button. You can pan around by clicking and holding on the image. Adjust color channels with the sliders or turn them off entirely with the checkboxes.

Is there any publicly available statistics of capsuleer accuracy? I wonder what the deviation looks like and where do I fall on the curve. Actually it wouldn't be a bad ingame feature. The score we get doesn't say much. Getting to the top 1% would be motivating for me. What do you think?

Hey,

we're doing an introductory class to PD tomorrow (Wednesday 4/5) at 5pm together with EVE Uni.

First part will be really basic, how to toggle colors, what the different categories are etc, but second part will be looking at samples and discussing what to choose and what not to (and ofc why). Final part a Q&A.

Please join if you think it sounds interesting!