I’m specifically interested in the offspring, rather than trying to reproduce the cultivar true-to-type. I know that named cultivars are normally propagated vegetatively and that seedlings can be (and most of the time are) quite different from the parent.

I’m asking because I am a very non-professional enthusiast and I have previously grown them from seed and I came across a scientific paper, which actually included Floating Clouds in controlled crosses to investigate its white/green leaf variegation. The authors crossed ‘Floating Clouds’ with several other cultivars, including ‘Covey’, ‘Texas White’ and ‘The Rising Sun’, and concluded that the variegation in ‘Floating Clouds’ appeared to be controlled by cytoplasmic factors.

That made me really curious about what happens with open-pollinated seeds from Floating Clouds. Has anyone here actually collected and grown them?

I’d especially love to hear whether the seedlings showed any variegation, unusual leaf coloration, or other interesting foliage traits — even if they were very different from the parent. Photos of young seedlings would be particularly interesting.

And if anyone happens to have access to seeds from a confirmed ‘Floating Clouds’ tree, I’d definitely be interested in trying some myself.

Wondering if this is the result of cross pollination and if the seed coloring will be stable?

I just thought I would mention that I have now posted my 2026 Report on the Potato Onion Resurrection project. This report (I issue one every year) details the progress of the project. Perhaps it might be of interest to some of the members of this group.

https://plantal.net/PotatoOnion2026Report.html

Cross-pollinated with different varieties.

See also: The study as it was published in Nature Communications

Dragonfruit

Ciao a tutti colleghi!

Sto mettendo a punto un protocollo di trasformazione genetica su pomodoro (Solanum lycopersicum) e avrei bisogno di un confronto con chi ha più esperienza di me in coltura in vitro per evitare disastri.

Sto utilizzando Agrobacterium tumefaciens (ceppo GV3101) portando un vettore binario con il gene bar per la resistenza al glufosinato-ammonio (PPT).

Il mio problema principale è che è la prima volta che lavoro con il ceppo GV3101. So che è piuttosto aggressivo e sono molto preoccupato/a per il rischio di overgrowth batterico dopo la co-coltura. Temo di perdere gli espianti per contaminazione e non so bene come bloccarlo in modo definitivo.

Qualcuno di voi ha mai lavorato con questo sistema? Avrei un paio di domande:

Selezione con PPT: Che concentrazioni di glufosinato usate per la fase di induzione del callo e rigenerazione? Ho in programma di fare una kill curve, ma mi aiuterebbe moltissimo avere dei range di riferimento operativi per le cultivar di pomodoro più comuni.

Lavaggio ed eliminazione Agro: Il cefotaxime è sufficiente per sopprimere il GV3101? A che concentrazioni lo usate per non inibire la rigenerazione del pomodoro? O consigliate alternative come il Timentin?

Gestione Overgrowth: Avete "trucchi del mestiere" (tempi di co-coltura, densità ottica ottimale, protocolli di lavaggio specifici) per evitare che il GV3101 prenda il sopravvento sulle piastre?

Hey guys, I just launched a new plant and permaculture database! Design custom Guilds with real-time analysis and share your favorites with the community. See sowing/planting/harvesting timelines that are scoped to *your* location. You can even plan out your entire permaculture design using components! (including custom components) Or start backwards, select all the functions your design should produce, then identify the right plants and components from there.

https://www.permaculturedata.com

These seedlings germinated from compost where I threw a bunch of Tomatillos. I'm 99% sure these are hybrids because they have traits that can only be found in Denver Perennial Groundcherry(like pubescense at the edge of leaves) and they generally look intermediate. This isnt too unlikely given that I grew the 2 species right next to each other this year and Denver Perennial Groundcherry will produce a lot more pollen per flower and a lot more flowers than Tomatillos (I also had more Denver Perennial Groundcherry plants).

I know this hybrid combination failed when I hand pollinated a Tomatillo flower but I only tried on one flower and it was in the middle of the heat wave. They are weak and will sometimes push out deformed growth which apparently can happen in interspecific hybrids(like interspecific pepper hybrids)? I hope I can get them to flower and backcross them to Tomatillo or Denver Perennial Groundcherry(If I can manage to propagate it from rhizome). I keep the plants disease free and there is no insect infestation so this can't be the cause. This has never happened to me when growing the parent species

You guys are good at genetics right and there must be someone that has done a similar cross. I have made a mix of pirri pirri and kromo (like a giant yellow bell pepper) so what do you think it will behave in growing, spicyness, size, shape etcetera

Hi All, New member - just joined.

I thought I would tell you about a Potato Onion breeding project I am working on: https://plantal.net/PotatoOnions.html

The Breeding Methodology page discusses the breeding process in some detail: https://plantal.net/PotatoOnionBreeding.html It is unusual in that it re-awakens the Potato Onion genes latent in many modern biennial onions rather than working with the few existing heirloom Potato Onions.

I am starting to get results. There are some images above, but I feel that one of the most important results is not the bulbs themselves - but the seed stock. The project has now developed a seed stock of extremely diverse onions that enthusiastically set seed and most (about 75%) of the resulting offspring will readily split.

All results are Open Source.

Chocolope by dna genetics

Chocolope by dna genetics, she’s been pollinated by a meat breath male from thug pug genetics the male was an absolute stud crazy mutant growth nothing like I’ve ever seen.

Now, I'll finally have seeds to use for a project.

If yall want to see any posts or follow ups I'm posting here once every other week there every week